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READMEDescription of the data file contents and methods.Open Data Commons Attribution (ODC-By) V1.0, Open Access
Open Data Commons Attribution (ODC-By) V1.0, Open Access
Open Data Commons Attribution (ODC-By) V1.0, Open Access
Dataset
Dataset Describing Biodegradation of Individual Polychlorinated Biphenyl Congeners (PCBs) by Paraburkholderia xenovorans LB400 in presence and absence of sediment slurry
University of Iowa
12/04/2020
DOI: 10.25820/data.006135
Abstract
This dataset describes the biodegradation of polychlorinated biphenyl (PCB) congeners by Paraburkholderia xenovorans LB400 in absence and presence of PCB-contaminated sediment slurry, over time (Bako et. al., In Review). These experiments were designed to further understand how biodegradation of PCB-degrading microorganisms can be limited by slow mass transfer of PCB molecules from the sediment particles to living cells. The data resulting from these experiments can inform future experimental design and be reused by other researchers for further analysis and / or interpretive insights.
Details
- Title: Subtitle
- Dataset Describing Biodegradation of Individual Polychlorinated Biphenyl Congeners (PCBs) by Paraburkholderia xenovorans LB400 in presence and absence of sediment slurry
- Creators
- Christian M. Bako - University of IowaTimothy E Mattes - University of Iowa, Civil and Environmental EngineeringRachel F Marek - University of Iowa, IIHR--Hydroscience and EngineeringKeri C Hornbuckle - University of Iowa, Civil and Environmental EngineeringJerald L Schnoor - University of Iowa, Civil and Environmental Engineering
- Resource Type
- Dataset
- Publisher
- University of Iowa
- DOI
- 10.25820/data.006135
- Locations
- Latitude: 37.114 Longitude: -79.272
- Language
- English
- Date published
- 12/04/2020
- Description methods
- In absence of sediment, PCBs were extracted from aqueous bioreactors by liquid-liquid extraction (LLE) with hexane. In presence of sediment, the extraction method used was a modification of U.S. EPA Method 3545. Sediment slurry samples were extracted from bioreactors using pressurized fluid extraction (Accelerated Solvent Extractor; Dionex ASE-200) with equal parts acetone and hexane. GC-MS/MS triple quadrapole technology in multiple reaction monitoring mode (MRM) was used for identification and quantification of 209 PCBs as 174 chromatographic peaks. Samples were processed in batches of five along with one method blank per batch. All materials used in sample extraction had either been triple rinsed with solvent (methanol, acetone, and hexane) or combusted overnight at 450°C to prevent background PCB contamination. Results from the method blanks were used to determine the limit of quantification (LOQ) as the upper limit of the 95% confidence interval (average mass plus two times the standard deviation). PCB congener masses were corrected for surrogate recoveries less than 100%. The PCB concentration dataset was dichotomized at the threshold of the congener specific LOQ. Concentrations of congeners below the LOQ were treated as zero.
- Academic Unit
- Iowa Superfund Research Program; Civil and Environmental Engineering; IIHR--Hydroscience and Engineering; Occupational and Environmental Health
- Record Identifier
- 9984002165902771
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