Imaging synaptic activity in intact brain and slices with FM1-43 in C. elegans, lamprey, and rat

A R Kay; A Alfonso; Simon Alford; H T Cline; A M Holgado; B Sakmann; V A Snitsarev; T P Stricker; M Takahashi; Ling-Gang Wu

doi:10.1016/S0896-6273(00)81029-6

Back

Imaging synaptic activity in intact brain and slices with FM1-43 in C. elegans, lamprey, and rat

Journal article

Open access

Peer reviewed

Imaging synaptic activity in intact brain and slices with FM1-43 in C. elegans, lamprey, and rat

A R Kay, A Alfonso, Simon Alford, H T Cline, A M Holgado, B Sakmann, V A Snitsarev, T P Stricker, M Takahashi and Ling-Gang Wu

Neuron (Cambridge, Mass.), Vol.24(4), pp.809-817

12/1999

DOI: 10.1016/S0896-6273(00)81029-6

PMID: 10624945

Files and links (1)

url

https://doi.org/10.1016/S0896-6273(00)81029-6View

Published (Version of record) Open Access

Abstract

The fluorescent probe FM1-43 has been used extensively for imaging vesicle recycling; however, high nonspecific adsorption resulting in elevated background levels has precluded its use in certain tissues, notably brain slices. We have found that a sulfobutylated derivative of beta-cyclodextrin (ADVASEP-7) has a higher affinity for FM1-43 than the plasma membrane. ADVASEP-7 was used as a carrier to remove FM1-43 nonspecifically bound to the outer leaflet of the plasma membrane or extracellular molecules, significantly reducing background staining. This has enabled us to visualize synaptic vesicle recycling in the nematode C. elegans, intact lamprey spinal cord, and rat brain slices.

Brain - anatomy & histology

Brain Stem - anatomy & histology

Rats, Wistar

Rats, Long-Evans

Male

Axons - physiology

Brain - physiology

Brain Stem - physiology

Drug Carriers

Caenorhabditis elegans - physiology

Brain - cytology

Lampreys - physiology

Pyridinium Compounds

Quaternary Ammonium Compounds

Synapses - physiology

Rats

Spinal Cord - anatomy & histology

Synapses - ultrastructure

Animals

Image Processing, Computer-Assisted

Spinal Cord - physiology

Liposomes

In Vitro Techniques

Cyclodextrins - pharmacology

Fluorescent Dyes

Details

Title: Subtitle: Imaging synaptic activity in intact brain and slices with FM1-43 in C. elegans, lamprey, and rat
Creators: A R Kay - Department of Biological Sciences, University of Iowa, Iowa City 52242, USA. alan-kay@uiowa.edu
A Alfonso
Simon Alford
H T Cline
A M Holgado
B Sakmann
V A Snitsarev
T P Stricker
M Takahashi
Ling-Gang Wu
Resource Type: Journal article
Publication Details: Neuron (Cambridge, Mass.), Vol.24(4), pp.809-817
Publisher: United States
DOI: 10.1016/S0896-6273(00)81029-6
PMID: 10624945
ISSN: 0896-6273
eISSN: 1097-4199
Language: English
Date published: 12/1999
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9983992038702771

Metrics

14 Record Views

129 Times Cited - Web of Science