Abstract
1020 THE ANTIGENIC DETERMINANTS OF VARICELLAZOSTER VIRUS
Pediatric research, Vol.15, pp.612-612
04/1981
DOI: 10.1203/00006450-198104001-01046
Abstract
Antigenicity and immunogenicity are important considerations in the laboratory evaluation of candidate varicella-zoster virus (VZV) vaccines. Therefore, high titered antisera were prepared in guinea pigs and rabbits against two VZV strains: VZV-32 (a laboratory strain) and VZV-Oka (a vaccine strain). Radiolabeled proteins and glycoproteins were precipitated from VZV-infected cells with the VZV immune sera, as well as human zoster sera, and analyzed by polyacrylamide gel electrophoresis. Three major glycopeptides of approximate mol. wt. 62,000, 98,000, and 118,000 were identified; two of the three glycoproteins have been found in the membranes of VZV-infected cells and in culture medium overlying VZV-infected cells. At least seven nonglycosylated antigens, which ranged in mol. wt. from ∼30,000 to 150,000, also were present in the precipitates. The prominent high mol. wt. antigen probably corresponds to the major capsid polypeptide found in herpesviruses. In summary, at least 10 polypeptides were consistently precipitated by VZV anti-sera obtained from hyperimmunized animals. Since both strains elicited the same spectra of VZV antibodies, they could not be distinguished from one another on the basis of their electrophoretic profiles.
Details
- Title: Subtitle
- 1020 THE ANTIGENIC DETERMINANTS OF VARICELLAZOSTER VIRUS
- Creators
- Charles Grose
- Resource Type
- Abstract
- Publication Details
- Pediatric research, Vol.15, pp.612-612
- DOI
- 10.1203/00006450-198104001-01046
- ISSN
- 0031-3998
- eISSN
- 1530-0447
- Language
- English
- Date published
- 04/1981
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093478702771
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