Abstract
516 Peripheral and tumoral immune activity in the expansion part of the first-in-human DuoBody®-PD-L1×4–1BB (GEN1046) trial
Journal for immunotherapy of cancer, Vol.9(Suppl 2), pp.A546-A546
11/10/2021
DOI: 10.1136/jitc-2021-SITC2021.516
Abstract
BackgroundDuoBody-PD-L1×4-1BB (GEN1046) is a class-defining, bispecific immunotherapy designed to induce an antitumor immune response by simultaneous and complementary PD-L1 blockade and conditional 4-1BB stimulation. Encouraging clinical activity and manageable safety were observed during dose escalation in the ongoing phase 1/2a trial in patients with advanced solid tumors (NCT03917381). We report exploratory pharmacodynamic analyses and potential biomarkers of response in an expansion cohort of patients with PD-(L)1–R/R NSCLC.MethodsPatients with metastatic/unresectable NSCLC who had multiple lines of prior systemic therapy, including a checkpoint inhibitor, received flat-dose DuoBody-PD-L1×4-1BB (100 mg) intravenously every 3 weeks. Immunophenotyping of peripheral blood and measurements of soluble immune mediators were evaluated in serial blood samples in cycles 1–2. Tumor PD-L1 and 4-1BB expression and additional immune markers were evaluated by immunohistochemistry in core needle tumor biopsy specimens collected before treatment and at cycle 2.ResultsAs of May 2021, 40 patients with PD-(L)1–R/R NSCLC were enrolled (median age, 63 years). Treatment with DuoBody-PD-L1×4-1BB elicited pharmacodynamic modulation of immune endpoints within the first 2 cycles. Induction of peripheral IFN-y, CXCL9/10, and expansion of peripheral CD8+ effector memory T cells and activated NK cells were observed starting at cycle 1 (>2-fold from baseline) and maintained or increased through cycle 2. Based on 9 paired tumor biopsy samples, increased PD-L1 and 4-1BB expression and cytotoxic CD8+/GZMB+ cell density were detected following treatment. In a subset of patients with clinical response (n=5 confirmed PRs), a trend of greater induction of IFN-y, CXCL9/10, and activated NK cells was observed vs nonresponders. Disease control rates were higher in patients who had progressed on prior anti–PD-1 therapy within 8 months (64% [16/25]) from the first dose of DuoBody-PD-L1×4-1BB. As expected, among patients with evaluable baseline tumors (n=26), most with any degree of tumor reduction (best change, <0%) harbored PD-L1+ tumors (≥1% tumor positive score; 7/10) and showed close spatial proximity between PD-L1+ and 4-1BB+ cells. Conversely, most patients without any degree of tumor reduction presented with PD-L1− tumors (12/16).ConclusionsIn patients with NSCLC who progressed on PD-(L)1 therapy, DuoBody-PD-L1×4-1BB elicited pharmacodynamic effects consistent with its proposed mechanism of action. Relationships between disease control and PD-L1 tumoral expression, as well as time from last prior anti–PD-1 therapy, were observed. These findings support that patient selection and/or anti–PD-1 combination therapy may lead to improved clinical efficacy. Further analyses are ongoing and updated results will be presented.AcknowledgementsThe authors thank Hrefna Kristin Johannsdottir, Lei Pang, and Kate Sasser at Genmab A/S and Friederike Gieseke at BioNTech SE for their valuable contributions. This trial was funded by Genmab A/S and BioNTech SE.Trial RegistrationNCT03917381Ethics ApprovalThis trial is undertaken following full approval of the final protocol, amendments, informed consent form, applicable recruiting materials, and subject compensation programs by the Independent Ethics Committee/Institutional Review Board.ConsentWritten informed consent, in accordance with principles that originated in the Declaration of Helsinki 2013, current ICH guidelines including ICH-GCP E6(R2), applicable regulatory requirements, and sponsor policy, was provided by the patients.
Details
- Title: Subtitle
- 516 Peripheral and tumoral immune activity in the expansion part of the first-in-human DuoBody®-PD-L1×4–1BB (GEN1046) trial
- Creators
- Santiago Ponce Aix - Hospital Universitario 12 De OctubreEmiliano CalvoVictor Moreno - Hospital Universitario Fundación Jiménez DíazElena Garralda - Vall d’Hebron Institute of Oncology, Barcelona, SpainAndrés Cervantes - Universitat de ValènciaSuresh Ramalingam - Emory and Henry CollegeJosé Trigo Pérez - Hospital Universitario Virgen de la Victoria, Málaga, SpainPatricia LoRusso - Yale Cancer CenterMuhammad Furqan - University of IowaDaniel Cho - Tisch HospitalAlexander Muik - BioNTechEleni Lagkadinou - BioNTechÖzlem Türeci - BioNTechSuzana Couto - GenmabNora Pencheva - GenmabUlf Forssmann - GenmabUğur Şahin - BioNTechTahamtan Ahmadi - GenmabBrandon Higgs - GenmabMaria Jure-Kunkel - GenmabIgnacio Melero - Clinica Universidad de Navarra
- Resource Type
- Abstract
- Publication Details
- Journal for immunotherapy of cancer, Vol.9(Suppl 2), pp.A546-A546
- Publisher
- BMJ Publishing Group Ltd
- DOI
- 10.1136/jitc-2021-SITC2021.516
- ISSN
- 2051-1426
- eISSN
- 2051-1426
- Language
- English
- Date published
- 11/10/2021
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984362335502771
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