Abstract
54313 Transient changes in the cutaneous microbiome after skin surgery
Journal of the American Academy of Dermatology, Vol.91(3 Supplement), pp.AB343-AB343
09/2024
DOI: 10.1016/j.jaad.2024.07.1367
Abstract
Background: While bacteria have traditionally been viewed as purely pathogenic organisms, recent insight into mutualism between the immune system and skin microbes shows their instrumental role in skin homeostasis1. Despite the growing interest in this field, little investigation exists analyzing the role of commensal microbes in cutaneous surgery.
Methods: A prospective cohort study in patients undergoing excision of non-melanoma skin cancers at the University of Iowa Hospitals and Clinics. Subjects underwent swabbing of the site for microbial shotgun metagenomic sequencing prior to site decontamination/excision of skin cancer (visit 1), again at 14-day follow-up for suture removal (visit 2) and during their first post-surgical follow-up at 3-6 months (visit 3).
Results: 18 subjects completed requirements sufficient for analysis. Propionibacteriaceae were most isolated with a median relative abundance of 19.7%, 5.7% and 22.8% at visits 1, 2 and 3, respectively. Corynebacteriaceae were significantly increased at visit 2 (29%), compared to visit 1 (4.68%, p=0.0017) and visit 3 (5.7%, p = 0.0042). Staphylococcus aureus had increased abundance at visit 2 (15.1%) compared to visit 1 (0.9%, p=0.0389) and visit 3 (0.3%, p=0.0126). Median alpha diversity was significantly decreased at visit 2 (Chao1 = 12) versus visits 1 (Chao1 = 65.5, p=0.0006) and visit 3 (Chao1 = 117.5, p < 0.001).
Conclusion: Surgical wounds in the acute phase of healing demonstrate transient changes in microbiome diversity. How these changes translate into clinical outcomes and how they may be optimized to improve wound healing merits further study.
Details
- Title: Subtitle
- 54313 Transient changes in the cutaneous microbiome after skin surgery
- Creators
- Trenton Greif - University of Iowa, Department of DermatologyKelly Messingham - University of Iowa, DermatologyVincent Liu - University of IowaJennifer Powers - University of Iowa
- Resource Type
- Abstract
- Publication Details
- Journal of the American Academy of Dermatology, Vol.91(3 Supplement), pp.AB343-AB343
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.jaad.2024.07.1367
- ISSN
- 0190-9622
- Language
- English
- Date published
- 09/2024
- Academic Unit
- Pathology; Dermatology
- Record Identifier
- 9984699047702771
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