Abstract
6.43 Epigenetic Evidence of Nascent Smoking Concentrated Among Low Income Youth
Journal of the American Academy of Child and Adolescent Psychiatry, Vol.56(10), pp.S291-S291
10/2017
DOI: 10.1016/j.jaac.2017.09.388
Abstract
Objectives
The goal of this presentation is to characterize smoking-associated demethylation of CpG cg05575921 in a high-risk cohort of adolescents.
Methods
Longitudinal cohort study comprised of 318 10th graders in eastern Iowa identified as being at high risk for smoking (subjects endorsed having a friend who smokes cigarettes). Serial measures include the Semi-Structured Assessment for the Genetics of Alcoholism, Adolescents, Version IV; body mass index (BMI); patient health questionnaire (PHQ-9) depression inventory; measurement of serum cotinine; carboxy-tetrahydrocannabinol (carboxy-THC) by enzyme-linked immunosorbent assay; and measurement of cg05575921 methylation by bisulfite conversion of whole-blood DNA and digital droplet polymerase chain reaction. Cross-sectional data from intake at age 15 years were used for the current study.
Results
Demethylation of cg05575921 was significantly associated with serum cotinine in an unadjusted linear regression model ( P < 0.01, R 2 = 0.04). An additional model that adjusted for covariates, including age, sex, race/ethnicity, income, BMI, PHQ-9 score, serum carboxy-THC, self-reported smoking, and exposure to family members and other adults who smoke ( P < 0.01, R 2 = 0.06) revealed that low income was the only independent predictor of demethylation of cg05575921 ( P < 0.05).
Conclusions
Demethylation of cg05575921 is a promising epigenetic biomarker for detection of current smoking status in high-risk youth, particularly those with lower household income. These results are the first demonstration of the increased risk of smoking among low income youth using an epigenetic approach. Serial measurement is expected to reveal a stronger relationship between serum cotinine and cg05575921 demethylation as this cohort’s cumulative exposure to smoke increases. Validation of this biomarker in adolescents may open the door to targeted, efficacious early interventions for adolescent smoking and have a significant public health impact in reducing the long-term harms of smoking, which disproportionately affect low-income populations.
Details
- Title: Subtitle
- 6.43 Epigenetic Evidence of Nascent Smoking Concentrated Among Low Income Youth
- Creators
- Allan M Andersen - University of IowaSamuel Kuperman - University of Iowa Carver College of MedicineJeff Long - University of IowaMeg Gerrard - University of ConnecticutRobert Philibert - University of Iowa
- Resource Type
- Abstract
- Publication Details
- Journal of the American Academy of Child and Adolescent Psychiatry, Vol.56(10), pp.S291-S291
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.jaac.2017.09.388
- ISSN
- 0890-8567
- eISSN
- 1527-5418
- Language
- English
- Date published
- 10/2017
- Academic Unit
- Psychiatry; Biostatistics; Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Stead Family Department of Pediatrics
- Record Identifier
- 9984071699902771
Metrics
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