959-101 Antioxidant Enzymes Attenuate Free Radical Generation After Direct Current Countershocks

Matthew R. Caterine; Kirk T. Spencer; Robin S. Smith; Garry R. Buettner; Richard E. Kerber

doi:10.1016/0735-1097(95)92331-X

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959-101 Antioxidant Enzymes Attenuate Free Radical Generation After Direct Current Countershocks

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959-101 Antioxidant Enzymes Attenuate Free Radical Generation After Direct Current Countershocks

Matthew R. Caterine, Kirk T. Spencer, Robin S. Smith, Garry R. Buettner and Richard E. Kerber

Journal of the American College of Cardiology, Vol.25(2), pp.210A-211A

02/1995

DOI: 10.1016/0735-1097(95)92331-X

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Abstract

Previous work in our lab has shown that direct current (DC) shocks generate free radicals, which may be toxic to the myocardium. We hypothesized that administration of free radical scavenging enzymes prior to shock delivery would attenuate the generation of free radicals. We used a newly developed and validated technique for real time quantitation of free radical production: ascorbyl free radical (AFR) concentration in coronary sinus blood, measured by electron paramagnetic resonance. In 10 open-chest dogs, 75J and 100J epicardial shocks were administered during NSR. Then superoxide dismutase (SOD, 15,000μ/kg).and catalase (CAT. 55,000 u/kg) were given as a continuous infusion. Ten mins. after initiating the SOD/CAT infusion, 75J and 100J shocks were repeated. Four control dogs received normal saline instead of SOD/CAT. % Δ AFRArea AFREnergyPre SODPost SODPre SODPost SOD75 J17±45±2*150±3937±8*100 J20±56±2*184±2653±17*x±SO.*p < 0,05 post vs. pre. Area=integrated area under AFR concentration vs. time curve The 4 control dogs showed no significant differences in AFR generation when comparing shocks administered before and after saline infusion rather than SOD/CAT. antioxidant enzymes markedly attenuate free radical generation by DC shocks. If free radicals are a mechanism of defibrillation injury in patients, drugs with free radical inhibiting or scavenging properties may prevent such injury.

Details

Title: Subtitle: 959-101 Antioxidant Enzymes Attenuate Free Radical Generation After Direct Current Countershocks
Creators: Matthew R. Caterine
Kirk T. Spencer
Robin S. Smith
Garry R. Buettner
Richard E. Kerber
Resource Type: Abstract
Publication Details: Journal of the American College of Cardiology, Vol.25(2), pp.210A-211A
Publisher: Elsevier Inc
DOI: 10.1016/0735-1097(95)92331-X
ISSN: 0735-1097
eISSN: 1558-3597
Language: English
Date published: 02/1995
Academic Unit: Radiation Oncology
Record Identifier: 9984314277802771

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