A model for the detection of pancreatic ductal adenocarcinoma circulating tumor cells

Matthew S. Alexander; Brianne R. O’Leary; Devon Moose; Juan Du; Michael D. Henry; Joseph J. Cullen

doi:10.14440/jbm.2018.250

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A model for the detection of pancreatic ductal adenocarcinoma circulating tumor cells

Abstract

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Peer reviewed

A model for the detection of pancreatic ductal adenocarcinoma circulating tumor cells

Matthew S. Alexander, Brianne R. O’Leary, Devon Moose, Juan Du, Michael D. Henry and Joseph J. Cullen

Journal of biological methods, Vol.5(3), pp.e97-e97

09/05/2018

DOI: 10.14440/jbm.2018.250

PMCID: PMC6706145

PMID: 31453247

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Abstract

Metastatic disease is the leading cause of pancreatic ductal adenocarcinoma (PDAC) associated death. PDAC cells invade and enter the bloodstream early, before frank malignancy can be detected. Our objective was to develop an in vivo assay enabling the identification and quantification of circulating tumor cells (CTCs) from primary orthotopic PDAC tumors. Human PDAC cells expressing luciferase and green fluorescent protein were orthotopically injected into the pancreas of mice utilizing ultrasound guidance. Bioluminescent imaging was conducted to identify and track tumor growth. CTCs were then isolated and analyzed by flow cytometry to detect GFP-expressing cancer cells. Tumor growth as measured by bioluminescent imaging increased over time. The concentration of CTCs correlated with the strength of bioluminescent imaging signal. In addition, livers bearing macroscopic disease were harvested for further imaging under fluorescence stereomicroscopy and confocal microscopy, which confirmed the presence of metastases. This study represents an orthotopic animal model that reliably detects the presence of CTCs from PDAC. There is a positive correlation between the concentrations of CTCs with overall tumor burden.

Metastasis

bioluminescent imaging

circulating tumor cells

orthotopic implantation

pancreatic adenocarcinoma

Details

Title: Subtitle: A model for the detection of pancreatic ductal adenocarcinoma circulating tumor cells
Creators: Matthew S. Alexander - University of Iowa Hospitals and Clinics
Brianne R. O’Leary - Departments of Surgery, , Departments of Molecular Physiology and Biophysics, Pathology and Urology, , Departments of Radiation Oncology
Devon Moose - University of Iowa
Juan Du - University of Iowa
Michael D. Henry - University of Iowa
Joseph J. Cullen - University of Iowa Hospitals and Clinics
Resource Type: Abstract
Publication Details: Journal of biological methods, Vol.5(3), pp.e97-e97
DOI: 10.14440/jbm.2018.250
PMID: 31453247
PMCID: PMC6706145
NLM abbreviation: J Biol Methods
ISSN: 2326-9901
eISSN: 2326-9901
Publisher: Journal of Biological Methods
Language: English
Date published: 09/05/2018
Academic Unit: Molecular Physiology and Biophysics; Pathology; Surgery; Radiation Oncology
Record Identifier: 9984302197002771

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