Abstract
Abstract 139: Ultrasound Stimulation Restores Impaired Neovascularization-Related Capacities of Human Circulating Angiogenic Cells
Circulation research, Vol.111(suppl_1)
08/03/2012
DOI: 10.1161/res.111.suppl_1.A139
Abstract
Abstract only
Aims:
Unsatisfactory effects of therapeutic angiogenesis in critical limb ischemia may be ascribed to use of circulating angiogenic cells (CACs) with impaired neovascularization-related capacities of atherosclerotic patients. We tested whether ultrasound cell stimulation can restore the impaired capacities.
Methods and Results:
During four-day-culture of human peripheral blood-derived mononuclear cells to achieve CACs, we stimulated the culture cells daily with low-intensity pulsed ultrasound stimulation (LIPUS). LIPUS to healthy volunteer-derived culture cells augmented generation and migration capacities of CACs, increased concentrations of angiopoietin 2 and nitrogen oxides in the culture medium, and increased expressions of phosphorylated-Akt and endothelial nitric oxide synthetase in CACs on western blotting. LIPUS to atherosclerotic patient-derived culture cells also augmented the generation and migration capacities of CACs. Although neovascularization in the ischemic hindlimb of athymic nude mice was impaired after intramuscular injection of atherosclerotic patient-derived CACs compared with that of healthy volunteer-derived CACs, LIPUS to atherosclerotic patient-derived culture cells restored the impaired neovascularization.
Conclusion:
Therapeutic angiogenesis with LIPUS-pretreated CACs may be a new strategy to rescue critical limb ischemia in atherosclerotic patients.
Details
- Title: Subtitle
- Abstract 139: Ultrasound Stimulation Restores Impaired Neovascularization-Related Capacities of Human Circulating Angiogenic Cells
- Creators
- Yasuyuki Toyama - Kurume UniversityKen-ichiro Sasaki - Kurume UniversityKatsuro Tachibana - Fukuoka UniversityTakafumi Ueno - Kurume UniversityHidemi Kajimoto - Kurume UniversityShinji Yokoyama - Kurume UniversityMsanori Ohtsuka - Kurume UniversityHiroshi Koiwaya - Kurume UniversityTakaharu Nakayoshi - Kurume UniversityYoshiaki Mitsutake - Kurume UniversityHidetoshi Chibana - Kurume UniversityNaoki Itaya - Kurume UniversityTsutomu Imaizumi - Kurume University
- Resource Type
- Abstract
- Publication Details
- Circulation research, Vol.111(suppl_1)
- DOI
- 10.1161/res.111.suppl_1.A139
- ISSN
- 0009-7330
- eISSN
- 1524-4571
- Language
- English; Japanese
- Date published
- 08/03/2012
- Academic Unit
- Cardiology; Stead Family Department of Pediatrics
- Record Identifier
- 9984961023302771
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