Abstract
Abstract 16716: Comparison of Blood Troponin I Complexes and Free Form in Acute Myocardial Infarction and End Stage Renal Disease
Circulation (New York, N.Y.), Vol.142(Suppl_3), pp.A16716-A16716
11/17/2020
DOI: 10.1161/circ.142.suppl_3.16716
Abstract
Abstract only
Introduction:
Circulating blood troponin complexes and free fractions remain poorly characterised in different conditions where troponin is detectable in blood
Hypothesis:
The aim of the study was to compare the differences in troponin-I(TnI) complexes/free-forms in end stage renal disease (ESRD) compared to acute myocardial infarction(AMI)
Methods:
Blood was collected from patients with AMI(n=7) or ESRD(n=4) at two time points (a)As early as possible after AMI or at initial contact with ESRD patients and (b)24-48 hours later. Western blotting was carried out with HyTest cTnI-560cc antibody on plasma extracted from whole blood. Densitometry analysis was performed and evaluated using the independent samples T-test and paired T-test as appropriate
Results:
Prominent bands were noted at ~45,~37 and ~25 kDa respectively representing low molecular weight(LMW) TnI-TnT-TnC complex, binary TnI-TnC complex and free-TnI. At time-point (a), there was no difference in these bands between STEMI and CKD patients. Interestingly, at time-point (b), AMI patients had significantly lower intensity of the 45kDa and 37kDa bands compared to CKD patients(for 45 kDa band mean difference was 54.3±19.4 AU, p=0.02; for 37 kDa band mean difference was 27.7±10.5 AU, p=0.03) as well as compared to the initial STEMI samples taken at time-point (a)(for 45 kDa band mean difference was 41.4±8.1 AU, p=0.002; for 37 kDa band mean difference was 16.7±6.3 AU, p=0.002) ,however there was no difference in the 25kDa band
Conclusions:
AMI patients had progressively lesser quantities of circulating LMW-ITC and binary IC complexes following AMI compared to ESRD patients, but similar quantities of circulating free TnI. This indicates a constant release of LMW-ITC and binary-IC complexes from the myocardium or reduced glomerular filtration of these complexes in ESRD while in the AMI patients, the LMW-ITC and binary I-C complexes appear to be progressively eliminated from plasma after the initial release
Details
- Title: Subtitle
- Abstract 16716: Comparison of Blood Troponin I Complexes and Free Form in Acute Myocardial Infarction and End Stage Renal Disease
- Creators
- Vikram Sharma - University of Iowa Hospitals and ClinicsRuchi Sharma - University of IowaShehab A Alansari - Cleveland ClinicMohan L Maradumane - Cleveland ClinicConner P Witherow - Cleveland ClinicWai Hong W Tang - Cleveland ClinicSathyamangala Prasad - Cleveland ClinicVenu Menon - Cleveland Clinic
- Resource Type
- Abstract
- Publication Details
- Circulation (New York, N.Y.), Vol.142(Suppl_3), pp.A16716-A16716
- DOI
- 10.1161/circ.142.suppl_3.16716
- ISSN
- 0009-7322
- eISSN
- 1524-4539
- Language
- English
- Date published
- 11/17/2020
- Academic Unit
- General Internal Medicine; Internal Medicine
- Record Identifier
- 9984693961302771
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