Abstract
Abstract 410: Advanced HR‐MRI Protocols for Ex‐Vivo Circle of Willis Imaging in 3T HR‐MRI
Stroke: vascular and interventional neurology, Vol.5
11/01/2025
DOI: 10.1161/svi270000_410
PMCID: PMC12850029
Abstract
Introduction There are a variety of embedding mediums used when imaging ex‐vivo specimens on high‐resolution MRI. The purpose of the current study is to compare three different methodologies to determine which medium will optimize the signal to noise ratio (SNR) and minimize artifact for ex‐vivo Circle of Willis (CoW) specimens. Methods CoW specimens were collected and temporarily stored in 10% neutral‐buffered formalin. To remove any residual blood clots, specimens were cleaned with Polysorbate 20 dissolved in distilled water. Then, specimens were stored in a solution of phosphate‐buffered saline with 0.05% sodium azide (Teknova, Hollister, CA USA) at 5 ° C. Shortly before imaging acquisition, specimens were cut into anterior and posterior/vertebrobasilar circulations. One third of the samples were embedded in a 2% agarose only solution, one third were placed into a Fomblin (a proton‐free fluid) only solution, and one third were embedded in an agarose solution which was then placed into a larger container filled with Fomblin. 3D T1‐weigted imaging was performed on a 3T high resolution GE SIGNA Premier MRI. To calculate SNR, two ∼0.15 mm3 region of interests (ROIs) were placed: one in the vessel wall of each specimen and another in the space surrounding the vessel. SNR was calculated as the mean signal intensity of the vessel wall ROI/ standard deviation of the background ROI. Results Three CoW specimens were imaged. The SNR was highest for the Fomblin only specimen (33.99) followed by the agarose‐embedded specimen surrounded by fomblin (27.90) and the agarose‐only specimen (15.93). Conclusion Fomblin appears to be the best imaging medium when imaging ex‐vivo CoW specimens. Figure caption: Ex‐vivo imaging of circle of willis in 3T HR‐MRI. (A) Imaging of a left middle cerebral artery using only agarose. (B) A basilar artery imaged using agarose embedded in fomblin. (C) A cross section image of a right middle cerebral artery using only fomblin.
Details
- Title: Subtitle
- Abstract 410: Advanced HR‐MRI Protocols for Ex‐Vivo Circle of Willis Imaging in 3T HR‐MRI
- Creators
- A. S GudinoE Baniewicz - University of IowaR Jaramillo - University of IowaM CabariqueA Van DamC Idrovo - University of Iowa, NeurologyL DiazN ShenoyC DierV MagnottaE. A Samaniego
- Resource Type
- Abstract
- Publication Details
- Stroke: vascular and interventional neurology, Vol.5
- DOI
- 10.1161/svi270000_410
- PMCID
- PMC12850029
- ISSN
- 2694-5746
- eISSN
- 2694-5746
- Publisher
- Wiley Subscription Services, Inc
- Language
- English
- Date published
- 11/01/2025
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Neurology; Radiology; Psychiatry; Iowa Neuroscience Institute; Neurosurgery
- Record Identifier
- 9985089135102771
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