Abstract
Abstract 4147419: EKG Abnormalities and Homozygous Embryonic Lethality in Gene-Targeted Mice Mimicking K1479 Acetylation of the Cardiac Na + Channel Na v 1.5
Circulation (New York, N.Y.), Vol.150(Suppl_1)
11/12/2024
DOI: 10.1161/circ.150.suppl_1.4147419
Abstract
Abstract only Introduction: Na v 1.5, the predominant Na + channel in cardiac myocytes, is encoded by SCN5A and is essential for depolarization and impulse propagation in the heart. Loss of function mutations of Na v 1.5 can lead to tachyarrhythmias, conduction disease and dilated cardiomyopathy by altering channel expression, kinetic properties, and/or membrane trafficking. We previously reported that amino acid K1479 of Na v 1.5 is acetylated, that K1479 acetylation or mutation to the acetylation mimic glutamine (K1479Q) decreases inward Na + current (I Na ) in heterologous expression systems by decreasing membrane trafficking, that cardiac myocytes from mice lacking the deacetylase Sirt1 (Sirt1 -/- ) are hyperacetylated and have less I Na , and that Sirt1 -/- mice have conduction disease and arrhythmias. Here, we studied the effect of the K1479Q mutation that mimics acetylation in-vivo . Methods: K1479Q knock-in mice were engineered via CRISPR/Cas9 on a C57BL6 background and backcrossed 4 times with C57BL6 wild type (WT) mice. Na v 1.5 WT/Q heterozygous (het) mice were mated, and male (M) and female (F) gene-targeted offspring and littermate controls were studied by electrocardiograms (EKGs) and echocardiograms (Echos) at 3 and 6 months of age. Data is shown as mean±SEM. Results: The K1479Q mutation was homozygous (hom) embryonic lethal (Na v 1.5 WT/Q x Na v 1.5 WT/Q crosses: WT, n=23; Het, n=49; Hom, n=0). On EKG (WT: n = 5M, 3F; Het: n = 6M, 5F), Na v 1.5 WT/Q mice had a prolonged PR interval at 3 months (42±1 vs. 36±1 ms, p=0.0003) and at 6 months (46±1 vs. 39±1 ms, p=<0.0001) compared to WT (Figure). Na v 1.5 WT/Q mice also showed a prolonged QTc interval (72±1 vs. 65±2 ms, p=0.007) compared to WT controls at 6 months. No significant differences were identified in cardiac size or function between Na v 1.5 WT/Q and WT mice at 6 months. Conclusions: The K1479Q Na v 1.5 mutation, which mimics the acetylation post-translational modification at K1479, leads to homozygous embryonic lethality and a heterozygous electrophysiological phenotype as severe as targeted deletion of the channel. These findings suggest that Na v 1.5-K1479Q and acetylated Na v 1.5-K1479 do not produce I Na in-vivo in cardiac myocytes, in part due to failure to traffic to the surface membrane.
Details
- Title: Subtitle
- Abstract 4147419: EKG Abnormalities and Homozygous Embryonic Lethality in Gene-Targeted Mice Mimicking K1479 Acetylation of the Cardiac Na + Channel Na v 1.5
- Creators
- Joseph Kaesbauer - University of Iowa, Internal MedicineJason Dierdorff - University of IowaAlexander Greiner - University of Iowa, Internal MedicineJin-Young Yoon - University of IowaKaikobad Irani - University of IowaBarry London - University of Iowa
- Resource Type
- Abstract
- Publication Details
- Circulation (New York, N.Y.), Vol.150(Suppl_1)
- Publisher
- LIPPINCOTT WILLIAMS & WILKINS
- DOI
- 10.1161/circ.150.suppl_1.4147419
- ISSN
- 0009-7322
- eISSN
- 1524-4539
- Language
- English
- Date published
- 11/12/2024
- Academic Unit
- Molecular Physiology and Biophysics; Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984749759702771
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