Abstract
Abstract 4365974: The MSX1-E135D Mutation Disrupts SUMOylation, Interactions with T-box Proteins, and Smooth Muscle Actin Expression
Circulation (New York, N.Y.), Vol.152(Suppl_3), pp.A4365974-A4365974
11/04/2025
DOI: 10.1161/circ.152.suppl_3.4365974
Abstract
Introduction: We previously reported a putative mutation in Muscle Segment Homeobox 1 (MSX1; p.E135D) in a multigenerational family with arrhythmogenic cardiomyopathy, myocardial fibrosis, valve disease, and sudden death. MSX1 is a transcriptional repressor that interacts with TBX3 and affects the second heart field, cardiac neural crest, and mesenchymal differentiation. Cardiac specific deletion of MSX1 in 4-week-old and 12-week-old mice caused high degree AV block, junctional tachyarrhythmia, and sinus exit block.
Questions: To study potential mechanisms by which MSX1-E135D could alter cardiac structure and function, we assessed its impact on 1) SUMOylation at a consensus SUMOylation site, MSX1-K133, 2) complex formation of MSX1 with T-box transcription factors, and 3) cardiac connective tissue.
Methods: For SUMOylation, MSX1-WT, MSX1-E135D, and MSX1-K15A-K133A were co-transfected with SUMO1 and PIAS1 in HEK293 cells. For T-box interactions, T-box transcription factors were co-transfected in HEK293 cells with FLAG-MSX1-WT or FLAG-MSX1-E135D and immunoprecipitated with anti-FLAG. For connective tissue, neonatal rat cardiac fibroblasts (NRCFs) were incubated with adenoviruses expressing MSX1-WT or E135D followed by TGF-beta (TGF-β) exposure and measurement of alpha smooth muscle actin (α-SMA).
Results: MSX1-WT but not MSX1-E135D could be SUMOylated on Western blot. MSX1-E135D co-immunoprecipitated TBX5 and TBX3 to a lesser degree than WT, while its interaction with TBX2 was unchanged. MSX1-E135D overexpression in neonatal rat cardiac fibroblasts (NRCFs) blocked TGF-β-induced upregulation of alpha smooth muscle actin (see Figure).
Conclusions: At the molecular level, MSX1-E135D appears to impair SUMOylation of MSX1 and weaken interactions with the T-box transcription factors, TBX3 and TBX5. At the developmental level, the mutation disrupts TGF-β pathway signaling, which could perturb the Endothelial to Mesenchymal Transition that drives developmental changes. These findings could underlie the myocardial fibrosis, arrhythmias, and valve disease seen in the family carrying the MSX1-E135D mutation.
Details
- Title: Subtitle
- Abstract 4365974: The MSX1-E135D Mutation Disrupts SUMOylation, Interactions with T-box Proteins, and Smooth Muscle Actin Expression
- Creators
- Alexander Greiner - University of IowaDiana Colgan - University of IowaKayla Henry - University of IowaKyle Current - University of IowaHaider Mehdi - University of IowaRebecca Gutmann - University of IowaBarry London - University of Iowa
- Resource Type
- Abstract
- Publication Details
- Circulation (New York, N.Y.), Vol.152(Suppl_3), pp.A4365974-A4365974
- DOI
- 10.1161/circ.152.suppl_3.4365974
- ISSN
- 0009-7322
- eISSN
- 1524-4539
- Publisher
- Lippincott Williams & Wilkins
- Language
- English
- Date published
- 11/04/2025
- Academic Unit
- Molecular Physiology and Biophysics; Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9985024257702771
Metrics
3 Record Views