Abstract
Abstract P251: Monitoring the Success of Smoking Cessation Using Dna Methylation Status at Cg05575921: Implications for Incentive Based Strategies for Quantitative Reduction of Harm
Circulation (New York, N.Y.), Vol.141(Suppl_1)
03/03/2020
DOI: 10.1161/circ.141.suppl_1.P251
Abstract
Cigarette smoking accounts for about 1/5 of all deaths from heart disease in the United States. Despite this grim fact, getting patients to quit smoking is extremely difficult. Encouragingly, several studies have shown that incentive based smoking cessation interventions may be more effective than traditional strategies. However, their implementation has been stymied by the lack of clinically implementable objective mechanisms to determine smoking cessation in those using nicotine replacement. Over the past several years, we and others have shown that DNA methylation status at a CpG site in the AHRR gene referred to as cg05575921 accurately classifies smoking status and predicts smoking intensity. In addition, we have developed a rapid clinically implementable digital PCR assay for assessing methylation at this locus using DNA prepared from blood or saliva. However, whether changes in DNA methylation at this locus occur quickly enough to allow the monitoring and possible incentivization of smoking cessation is unknown. To examine these possibilities, we followed 70 regular adult smokers as they underwent smoking cessation using a financial incentive based, pharmacologically assisted paradigm that stipulated no use of nicotine replacement. Consistent with prior reports, we found that as quantified by nondetectable serum cotinine levels at 1 month, 2 months and 3 months, 30% of all subjects who attempted to quit smoking in order to receive a $400 reward succeeded in quitting smoking. Over the course of their cessation trajectory, cg05575921 methylation demonstrated a stepwise increase commiserate with initial smoking intensity. Overall, the heaviest smokers, manifested a stepwise 14% increase in cg05575921 methylation while the lightest smokers manifested a 5% increase in cg05575921 methylation over the three month follow up period. Taken together with prior data, these new data demonstrate a robust reversion of cg05575921 in response to smoking cessation whose values can be used as objective evidence of smoking cessation even in those using nicotine replacement. In addition, because this method can detect and quantify partial cessation, these findings further suggest that the use of this technology together with incentive based approaches may also be useful in encouraging quantitative reduction of harm for cancer and smoking related cardiovascular risk in those who can reduce, but cannot fully quit smoking.
Details
- Title: Subtitle
- Abstract P251: Monitoring the Success of Smoking Cessation Using Dna Methylation Status at Cg05575921: Implications for Incentive Based Strategies for Quantitative Reduction of Harm
- Creators
- Robert Philibert - University of IowaMeesha Dogan - Univ of Iowa, Iowa City, IAEric Hoffman - University of Iowa
- Resource Type
- Abstract
- Publication Details
- Circulation (New York, N.Y.), Vol.141(Suppl_1)
- DOI
- 10.1161/circ.141.suppl_1.P251
- ISSN
- 0009-7322
- eISSN
- 1524-4539
- Language
- English
- Date published
- 03/03/2020
- Academic Unit
- Iowa Neuroscience Institute; Psychiatry; Roy J. Carver Department of Biomedical Engineering; Internal Medicine; Radiology
- Record Identifier
- 9984320007302771
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