Abstract
Abstract TP334: A Novel Murine Model of the Effects of Helicopter Emergency Medical Services Transportation on Ischemia and Thrombolysis
Stroke (1970), Vol.50(Suppl_1)
02/2019
DOI: 10.1161/str.50.suppl_1.TP334
Abstract
Background: Helicopter emergent medical services (HEMS) transportation is critical for rapid transportation of patients for mechanical thrombectomy and rtPA-mediated reperfusion. However, the effect of the multiple unique physical factors present in HEMS on the outcomes of these procedures is not known. Experimental models of ischemia-reperfusion that replicate HEMS conditions are needed in order to address this issue before putative neuroprotective therapies during flight trials can be accurately evaluated. Methods: A novel experimental model of “drip & ship” during HEMS was developed using middle cerebral artery occlusion (MCAO) with autologous clot in mice and an Mi2 helicopter adapted for animal research. Since vibration is one of the most salient physical components in HEMS, we isolated this factor for mechanistic investigation. The vibratory signature during HEMS was recorded in-flight using accelerometers and then recapitulated in a six-degree-of-freedom man-rated Moog-FCS motion platform in the laboratory. We evaluated two approaches (simultaneous vs. sequential) to expose mice with MCAO to the three different settings (helicopter/simulator/ground) in order to find the most optimal methodologically. Results: A clot length of 30 mm and 90 min of rTPA initiation post MCAO achieved significant infarctions while minimizing periprocedural mortality. Two MCAOs were the maximum capable of achieving occlusion times <15 minutes apart. In order to maintain MCAO to rtPA times close to 90 minutes, a sequential exposure to the three settings (helicopter/simulator/ground) was established with 2 mice each. Randomly assigned exposure lasted for 1 h and occurred at the same time of the rtPA exposure. The three exposures were 2 hours apart to minimize the effect of atmospheric variations. The animal MRI facility was coordinated to acutely scan the animals after the three exposures. Conclusion: We have developed an animal model to study the effects of HEMS on rtPA/reperfusion. An ongoing experiment measuring infarction volume and clinical outcomes will determine the need to maintain an actual HEMS for future experiments, or whether a laboratory simulator will suffice.
Details
- Title: Subtitle
- Abstract TP334: A Novel Murine Model of the Effects of Helicopter Emergency Medical Services Transportation on Ischemia and Thrombolysis
- Creators
- Nirav Dhanesha - University of IowaThomas Schnell - University of IowaSalam Rahmatalla - University of IowaJonathan DeShaw - University of IowaBradley Parker - University of IowaAnil Chauhan - University of IowaAndrew Pieper - University of IowaEnrique Leira - University of Iowa
- Resource Type
- Abstract
- Publication Details
- Stroke (1970), Vol.50(Suppl_1)
- DOI
- 10.1161/str.50.suppl_1.TP334
- ISSN
- 0039-2499
- eISSN
- 1524-4628
- Language
- English
- Date published
- 02/2019
- Academic Unit
- Neurology; Electrical and Computer Engineering; Psychiatry; Iowa Technology Institute; Injury Prevention Research Center; Roy J. Carver Department of Biomedical Engineering; Civil and Environmental Engineering; Occupational and Environmental Health; Hematology, Oncology, and Blood & Marrow Transplantation; Epidemiology; Iowa Neuroscience Institute; Industrial and Systems Engineering; Public Policy Center (Archive); Neurosurgery; Internal Medicine
- Record Identifier
- 9984186944002771
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