Abstract
Abundance of DNase-Susceptible Bacterial DNA from Damaged Cells in the Lungs of Pigs
Annals of the American Thoracic Society, Vol.11(1), pp.S72-S72
01/01/2014
DOI: 10.1513/AnnalsATS.201307-235MG
Abstract
Massively parallel DNA sequencing data suggest the presence of a diverse bacterial community in healthy lungs, yet, dogma dictates that immune mechanisms keep lungs sterile. Whether all bacterial DNA in bronchoalveolar lavage (BAL) corresponds to live bacteria or equals diversity in lung parenchyma is unknown. The authors have developed a DNase treatment-based method for bacterial DNA processing that discerns between intact cell DNA and DNA from bacteria with damaged cell walls. They investigated bacterial DNA load and diversity in transoral BAL samples and in transbronchial BAL and lung tissue samples obtained under sterile conditions in 6-week-old pigs. They hypothesized that bacterial DNA would correspond to dead bacteria and/or sample contamination from mouth bacteria and that lung tissue would be sterile.
Details
- Title: Subtitle
- Abundance of DNase-Susceptible Bacterial DNA from Damaged Cells in the Lungs of Pigs
- Creators
- Alejandro PezzuloBoulos NassarNicholas GansemerCedric RutlandDavid StoltzJoseph Zabner
- Resource Type
- Abstract
- Publication Details
- Annals of the American Thoracic Society, Vol.11(1), pp.S72-S72
- DOI
- 10.1513/AnnalsATS.201307-235MG
- ISSN
- 2329-6933
- eISSN
- 2325-6621
- Language
- English
- Date published
- 01/01/2014
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Iowa Neuroscience Institute; Internal Medicine
- Record Identifier
- 9984303003302771
Metrics
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