Abstract
Antioxidant protein levels and activities in epidermal progenitor cells
The FASEB journal, Vol.21(5), pp.A36-A36
04/2007
DOI: 10.1096/fasebj.21.5.A36
Abstract
The epidermis is a continuously renewing epithelium that is maintained by the presence of stem/progenitor cells. Besides the well known pathophysiological impact of UV‐induced oxidative stress in cutaneous malignancy, redox state may play a role in basal homeostasis of the epidermis, and particularly in stem/progenitor cell differentiation. However, no data existed until now, because isolating epidermal stem/progenitor cells was problematic. We used our previously reported method to separate stem/progenitor cells from their progeny (transient amplifying cells, or TA), and measured the levels of several antioxidant enzymes. Using Western blot analysis, copper‐zinc superoxide dismutase (SOD) was not detected in any murine extracts. Furthermore, glutathione peroxidase was equally present in all groups. However, the levels of manganese SOD and catalase were decreased in the progenitor cell population, compared to the total epidermal population and the TA cells. These data were confirmed by enzymatic activity assays, showing that catalase activity was decreased in stem/progenitor cells compared to the TA cells. These results suggest that different levels of reactive oxygen species are present in these cells. These data support the view that the antioxidant defense of stem/progenitor cells was decreased compared to more differentiated cells, and is consistent with their slow cycling property.
Details
- Title: Subtitle
- Antioxidant protein levels and activities in epidermal progenitor cells
- Creators
- Martine Dunnwald - University of IowaWanakee Carr - University of IowaLarry W Oberley - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.21(5), pp.A36-A36
- DOI
- 10.1096/fasebj.21.5.A36
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Publisher
- Federation of American Societies for Experimental Biology
- Number of pages
- 1
- Language
- English
- Date published
- 04/2007
- Academic Unit
- Anatomy and Cell Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984288643102771
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