Autoregulatory CD8 T cells induced by Listeria-encoded neuroantigen require B cells for functional suppression of EAE 9368

Connor R. Wilhelm; Nitin J. Karandikar; Alexander W. Boyden

doi:10.1093/jimmun/vkaf283.2779

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Autoregulatory CD8 T cells induced by Listeria-encoded neuroantigen require B cells for functional suppression of EAE 9368

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Autoregulatory CD8 T cells induced by Listeria-encoded neuroantigen require B cells for functional suppression of EAE 9368

Connor R. Wilhelm, Nitin J. Karandikar and Alexander W. Boyden

The Journal of immunology (1950), Vol.214(Supplement_1), vkaf2832779

11/01/2025

DOI: 10.1093/jimmun/vkaf283.2779

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Abstract

Abstract Description Myelin-reactive CD8 T cells (CD8s) have unexpected regulatory potential in MS and its mouse model experimental autoimmune encephalomyelitis (EAE). Previous work studied endogenous generation of these autoregulatory CD8s in mice, where infection with an intracellular bacterium Listeria monocytogenes engineered to express a myelin proteolipid protein sequence (LM-PLP) robustly protected from EAE. This protection was dependent on CD8s. Interestingly, reports suggest B cells aid in CD8 memory formation against LM. We hypothesized that B cells may influence induction of autoregulatory CD8s by LM-PLP. Here, we show that infection with LM-PLP fails to protect against EAE in mice lacking B cells and demonstrate that adoptive transfer of CD8s sourced from LM-PLP infected donors robustly protect recipients from EAE but fails to do so if donor mice lacked B cells. These preliminary results highlight a critical role for B cell:CD8 T cell interactions in this model. Ongoing experiments are characterizing these interactions (whether direct or indirect), phenotyping CD8s, and identifying potential B cell mechanisms influencing LM-PLP induced, EAE-suppressive, autoregulatory CD8 T cells. Topic Categories Immune Response Regulation: Cellular Mechanisms (IRC)

Animals - Rodent Cells - B Cells Diseases - Autoimmunity EAE/MS Processes - Inflammation

Details

Title: Subtitle: Autoregulatory CD8 T cells induced by Listeria-encoded neuroantigen require B cells for functional suppression of EAE 9368
Creators: Connor R. Wilhelm - University of Iowa
Nitin J. Karandikar
Alexander W. Boyden
Resource Type: Abstract
Publication Details: The Journal of immunology (1950), Vol.214(Supplement_1), vkaf2832779
DOI: 10.1093/jimmun/vkaf283.2779
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: Oxford University Press
Alternative title: IMMUNOLOGY2025™ Abstracts
Language: English
Date published: 11/01/2025
Academic Unit: Pathology
Record Identifier: 9985035033702771

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