Abstract
Cholinergic Mediated Renal Sodium Retention in Young Spontaneously Hypertensive Rats
The FASEB journal, Vol.33(S1), pp.861.2-861.2
04/2019
DOI: 10.1096/fasebj.2019.33.1_supplement.861.2
Abstract
Cholinergic activation with systemic nicotine infusion induces renal inflammation and the premature development of hypertension in young pre‐hypertensive spontaneously hypertensive rats (SHR), a genetic model of essential hypertension. We hypothesized that the nicotine induced inflammatory response is associated with renal sodium retention in the young SHR. Young pre‐hypertensive SHR and age matched Wistar Kyoto (WKY) controls received subcutaneous infusion of either saline (n=10 for each strain) or nicotine (15mg/kg/day) (n=10 for each strain) for 14 days. Animals received normal chow for the first 7 days of infusion and were changed to either a “low‐salt” (LSD, 0.01% NaCl) (n=10) or “high‐salt” (HSD, 4% NaCl) (n=10) diet. Animals were maintained in metabolic cages during the LSD and HSD periods. Nicotine did not alter sodium excretion significantly in WKY fed LSD or HSD, nor SHR fed LSD. In contrast, nicotine induced a 24% increase in renal sodium retention in SHR fed HSD, compared to controls (2.0±0.1 mM/gm vs 2.6±0.7mM/gm) (p<0.01). Moreover, nicotine‐induced sodium retention in young SHR fed HSD was associated with an increase in renal gene expression of serum & glucocorticoid kinase (SGK1) by 34% (p<0.033) and renal Na‐K‐Cl cotransporter (NKCC2) by 58% (p<0.01), compared to controls. Similarly, there was a 20% increase in the full length form (85kDa) of the alpha subunit of epithelial sodium channel (αENaC) 20% (p<0.05); however, no increase in the cleaved form (25kDa) of αENaC was observed, when compared to controls. No significant change in renal expression of the thiazide‐sensitive sodium‐chloride co‐transporter (NCC) was noted in response to nicotine when young SHR were fed HSD. We conclude that cholinergic mediated hypertension in the young SHR may be a form of salt‐sensitive hypertension, possibly related to renal inflammation and supported by renal sodium retention, and increased expression of renal sodium transporters.
Support or Funding Information
NIH 5K08HL119588‐02; Foundation de Leduq
Nicotine Induces Renal Sodium Retention in Young Pre‐hypertensive Spontaneously Hypertensive Rats (SHR) on a High‐Salt Diet (HSD).
This is from the Experimental Biology 2019 Meeting. There is no full text article associated with this published in The FASEB Journal.
Details
- Title: Subtitle
- Cholinergic Mediated Renal Sodium Retention in Young Spontaneously Hypertensive Rats
- Creators
- Nandita Raikwar - University of IowaAl Ryan - University of IowaPeter M Snyder - Veterans Affairs Medical CenterRobert Fenton - Aarhus UniversityFrancois Abboud - University of IowaSailesh Harwani - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.33(S1), pp.861.2-861.2
- Publisher
- The Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.2019.33.1_supplement.861.2
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Grant note
- NIH (5K08HL119588‐02) Foundation de Leduq
- Language
- English
- Date published
- 04/2019
- Academic Unit
- Molecular Physiology and Biophysics; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine; Medicine Administration
- Record Identifier
- 9984362737302771
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