Abstract
Comparative Profiling of the Immune System in Sarcoidosis via CITE-Seq and Flow Cytometry
The Journal of immunology (1950), Vol.204(1_Supplement), pp.224-224.24
05/01/2020
DOI: 10.4049/jimmunol.204.Supp.224.24
Abstract
Abstract CITE-Seq enables simultaneous single cell transcriptome and proteome analysis via combining single cell RNA-seq with oligo-labeled antibodies. Conventional techniques such as flow or mass cytometry have caveats including the number of epitopes accurately detected or an inability to recover samples for transcriptome analysis. These limitations are prohibitive for multivariate analysis of limited clinical samples. We developed a CITE-seq assay that enables comprehensive immune cell profiling of Sarcoidosis. Sarcoidosis is a granulomatous lung disease characterized by abnormal CD4+ T cell Th1 activity. However, the disease etiology and course are variable and the underlying molecular drivers remain unknown. The long-term goal of this study is to utilize CITE-seq to identify immune molecular pathways of Sarcoidosis pathogenesis. In our initial studies, we analyzed PBMC’s by CITE-seq vs. flow cytometry and observed similar cell profiles. However, the synergy of protein detection coupled with transcriptome analysis via CITE-seq enhanced cell subset identification vs. flow or scRNA-seq alone. We utilized CITE-seq in an ongoing longitudinal study of Sarcoidosis subjects to enhance resolution of the immune components contributing to disease. We compared CITE-seq to a flow cytometry panel analyzing the differential contributions of various CD4+ T cell lineages. The enhanced granularity provided by CITE-seq elucidated molecular pathways associated with disease pathogenesis. Thus, moving forward CITE-seq can provide the resolution and multivariate data collection required to identify the inflammatory drivers of Sarcoidosis.
Details
- Title: Subtitle
- Comparative Profiling of the Immune System in Sarcoidosis via CITE-Seq and Flow Cytometry
- Creators
- Roman E. Magallon - National Jewish HealthJennifer R. Knapp - National Jewish HealthLaura D. Harmacek - National Jewish HealthTing-Hui Clair Tu - National Jewish HealthBrian Vestal - National Jewish HealthMay Gillespie - National Jewish HealthKristyn MacPhail - National Jewish HealthLi Li - National Jewish HealthJill Elliot - National Jewish HealthBriana Barkes - National Jewish HealthLisa Maier - National Jewish HealthAnne Sommer - 3Dept. of Medicine, UCSFPineet Grewal - 3Dept. of Medicine, UCSFLaura Koth - 3Dept. of Medicine, UCSFNicholas Arger - 3Dept. of Medicine, UCSFBrenda Werner - University of IowaLinda Powers - University of IowaNabeel Hamzeh - University of IowaLinda Breslin - Johns Hopkins MedicineEdward Chen - Johns Hopkins MedicineThomas Danhorn - National Jewish HealthSonia M Leach - National Jewish HealthTasha E. Fingerlin - National Jewish HealthBrian P. O’Connor - National Jewish Health
- Resource Type
- Abstract
- Publication Details
- The Journal of immunology (1950), Vol.204(1_Supplement), pp.224-224.24
- DOI
- 10.4049/jimmunol.204.Supp.224.24
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 05/01/2020
- Academic Unit
- Internal Medicine; Pulmonary, Critical Care, and Occupational Medicine
- Record Identifier
- 9984362354902771
Metrics
4 Record Views