Abstract
Comprehensive identification of micropeptides encoded by long noncoding RNAs in human tissues
The FASEB journal, Vol.33(S1), pp.714.1-714.1
04/2019
DOI: 10.1096/fasebj.2019.33.1_supplement.714.1
Abstract
Many poorly characterized long noncoding RNAs (lncRNA) are expressed in human pancreas. Recently, a few lncRNAs have been found encode very small open reading frame, so‐called micropeptides. By regulating larger protein complex, these micropeptides have been shown to have important roles in fundamental biological processes such as muscle performance, mTORC1 activation and cell movement. To determine if any micropeptide play critical role for pancreas functions, we have took a bioinformatics approach to comprehensively analyze protein coding potential for lncRNAs expressed in human pancreas. We found more than 30 lncRNAs that are likely encode micropeptides. We have verified one conserved micropeptide, which we named Beta cell Glucose Regulated Micropeptide 1(BGRM1). We found BGRM1 regulate calcium homoeostasis and insulin secretion in beta cell. We have also performed mass spec to identify its binding proteins. Our result shows that some of lncRNA expressed in human pancreas might actually be translated as micropeptides and they play important roles for pancreas functions. We have also used the same pipeline to analysis lncRNA expressed in other organs. These results provides foundations for future comprehensive analysis of micropeptides functions.
This is from the Experimental Biology 2019 Meeting. There is no full text article associated with this published in The FASEB Journal.
Details
- Title: Subtitle
- Comprehensive identification of micropeptides encoded by long noncoding RNAs in human tissues
- Creators
- Huojun Cao - University of IowaFan Shao - University of IowaMark Li - University of IowaMason Sweat - University of IowaQingwen Qian - University of IowaYuwei Guo - University of IowaBrad Amendt - University of IowaLing Yang - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.33(S1), pp.714.1-714.1
- DOI
- 10.1096/fasebj.2019.33.1_supplement.714.1
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Publisher
- The Federation of American Societies for Experimental Biology
- Number of pages
- 1
- Language
- English
- Date published
- 04/2019
- Academic Unit
- Orthodontics; Molecular Physiology and Biophysics; Anatomy and Cell Biology; Endodontics; Craniofacial Anomalies Research Center; Fraternal Order of Eagles Diabetes Research Center; Dental Research
- Record Identifier
- 9984288759002771
Metrics
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