Abstract
Critical role of TRIF in modulating innate and adaptive responses during Citrobacter rodentium infection. (P4037)
The Journal of immunology (1950), Vol.190(1_Supplement), pp.131-131.19
05/01/2013
DOI: 10.4049/jimmunol.190.Supp.131.19
Abstract
Abstract Citrobacter sp. is a gram negative coliform facultative bacteria that is mostly associated with the inflammation of the gut and is widely studied as the mouse model organism for studying colitis. We previously showed that Citrobacter rodentium induces non-canonical inflammasome activation through a TRIF-dependent mechanism, however the exact role of TRIF during C. rodentium induced colitis is still unclear. Herein, we have used C. rodentium induced colitis model to study the role of TRIF in vivo and showed that TRIF plays a critical role in protection during C. rodentium induced colitis. Absence of TRIF results in increased bacterial burden and morbidity, which is dramatically increased when TRIF is absent in a Myd88-deficient background. TRIF-deficiency resulted in increased neutrophil numbers in the spleen. Similarly, dendritic cells frequency and numbers were dramatically increased and displayed activated phenotype. T cell frequencies and numbers were similar, however TNF-alpha producing CD4+ and CD8+ T cells were dramatically reduced. Further studies are being carried out in the lab to understand how TRIF regulates these various populations and functions of the innate and adaptive cells.
Details
- Title: Subtitle
- Critical role of TRIF in modulating innate and adaptive responses during Citrobacter rodentium infection. (P4037)
- Creators
- Prajwal Gurung - St. Jude Children's Research HospitalThirumala Kanneganti - St. Jude Children's Research Hospital
- Resource Type
- Abstract
- Publication Details
- The Journal of immunology (1950), Vol.190(1_Supplement), pp.131-131.19
- DOI
- 10.4049/jimmunol.190.Supp.131.19
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 05/01/2013
- Academic Unit
- Infectious Diseases; Internal Medicine
- Record Identifier
- 9984362347702771
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