Abstract
Development of an Injectable Cell-laden Microniche for Mesenchymal Stem Cell Therapy
Tissue engineering Part A, Vol.21(S1)
08/28/2015
DOI: 10.1089/ten.tea.2015.5000.abstracts
Abstract
Mesenchymal stem cells (MSCs) have shown promise in treatment of diabetic wounds, critical limb ischemia, and myocardial infarction by modulating local immune responses, promotion of tissue regeneration, and angiogenesis. This is accomplished through secreted and non-secreted factors such as VEGF, PGE2, TGF-β, and IDO. Currently, delivery of MSCs is limited by poor short-term engraftment, survival, and maintenance of cell potency. In this study, we examine the effects of injectable biodegradable cell microcarriers on MSCs' trophic properties. We have developed two systems of injectable biodegradable microcarriers: one composed of poly(lactide co-glycolide) (PLGA) and the other created from cross-linked gelatin. Both microcarriers systems were synthesized using a single emulsion process. The PLGA microcarriers were surface modified with fibronectin to promote cell attachment and the gelatin microcarriers were stabilized for use in physiological conditions by cross-linking with genipin at elevated temperatures. Dozens of MSCs quickly populate each carrier, creating an injectable cell-laden microcarrier. MSCs are stable and viable on the carriers for greater than five days providing a platform on which MSCs can be grown and then directly injected. These biodegradable injectable cell-laden microcarriers have the potential to promote superior control of MSC phenotype, viability, and maintenance of cell function post injection.
This study was supported by the European Research Council Advanced Grant (ERC-2012-AdG_20120216-321266) for the project ComplexiTE.
Details
- Title: Subtitle
- Development of an Injectable Cell-laden Microniche for Mesenchymal Stem Cell Therapy
- Creators
- Anthony J Burand Jr - University of IowaA J Brown - University of IowaJames A Ankrum - University of Iowa, Roy J. Carver Department of Biomedical Engineering
- Resource Type
- Abstract
- Publication Details
- Tissue engineering Part A, Vol.21(S1)
- Publisher
- Mary Ann Liebert, Inc. publishers
- DOI
- 10.1089/ten.tea.2015.5000.abstracts
- ISSN
- 1937-3341
- Language
- English
- Date published
- 08/28/2015
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984001795102771
Metrics
18 Record Views