Abstract
Effects of recurring intraocular pressure elevations on the retina and the autoimmune component
Acta ophthalmologica (Oxford, England), Vol.93(S255), p.n/a
10/2015
DOI: 10.1111/j.1755-3768.2015.0607
Abstract
Purpose
In glaucoma, complex changes of IgG serum autoantibody (Aab) repertoire highlight the possible contribution of an autoimmune component in the pathogenesis of this neurodegenerative disease. An elevated intraocular pressure (IOP) and its fluctuations are considered as major risk factors. The aim of the study was to identify the systemic influence of fluctuations using recurring pressure peaks and drops in a glaucoma animal model.
Methods
Sedated male Long Evans rats experienced a unilateral, intermittent IOP manipulation using a silicone loop adjusted around the eye globe for 1 hour during 27 treatments. The pressure profile included pressure peaks to 35 and 45 mmHg and drops to a physiologic value of 8 mmHg. Contralateral (n = 12) and untreated eyes (n = 14) served as controls. Neurodegeneration was determined after paraphenylenediamine staining and Brn3a staining. Microglia activation was identified after Iba1 staining in retinal cross‐sections. Changes of serum immunoreactivities were identified using a microarray approach with a glaucoma‐specific antigen Setup.
Results
A loss of axon density (as axons/0.05 mm2) of treated eyes (19624 ± 1709) compared to contralateral (21943 ± 1510; p < 0.01) and untreated eyes (22267 ± 1408; p < 0.01) occured, which was confirmed by retinal ganglion cell count. Next to an activation of microglia, upregulated Aab reactivities for GST, transferrin, and NSE were identified in serum of treated animals in comparison to control serum.
Conclusions
This animal model including pressure peaks and drops allows the establishment of individual IOP profiles. The retina and the optic nerve sustained a significant damage and an altered autoimmune repertoire could be induced. A significant influence of recurring pressure fluctuations could be demonstrated using this sophisticated glaucoma animal model.
Details
- Title: Subtitle
- Effects of recurring intraocular pressure elevations on the retina and the autoimmune component
- Creators
- J Teister - Experimental OphthamologyO Gramlich - Experimental OphthamologyM Neumann - Experimental OphthamologyX Tao - Experimental OphthamologyN Pfeiffer - Experimental OphthamologyF Grus - Experimental Ophthamology
- Resource Type
- Abstract
- Publication Details
- Acta ophthalmologica (Oxford, England), Vol.93(S255), p.n/a
- DOI
- 10.1111/j.1755-3768.2015.0607
- ISSN
- 1755-375X
- eISSN
- 1755-3768
- Number of pages
- 1
- Language
- English
- Date published
- 10/2015
- Academic Unit
- Iowa Neuroscience Institute; Neuroscience and Pharmacology; Ophthalmology and Visual Sciences
- Record Identifier
- 9984071613502771
Metrics
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