Abstract
Electrolyte transport properties in distal small airways from cystic fibrosis pigs with implications for host defense.(Report)(Author abstract)
American journal of physiology. Lung cellular and molecular physiology, Vol.310(4), p.L670
04/01/2016
DOI: 10.1152/ajplung.00422.2015.
Abstract
While pathological and clinical data suggest that small airways are involved in early cystic fibrosis (CF) lung disease development, little is known about how the lack of cystic fibrosis transmembrane conductance regulator (CFTR) function contributes to disease pathogenesis in these small airways. Large and small airway epithelia are exposed to different airflow velocities, temperatures, humidity, and C[O.sub.2] concentrations. The cellular composition of these two regions is different, and small airways lack submucosal glands. To better understand the ion transport properties and impacts of lack of CFTR function on host defense function in small airways, we adapted a novel protocol to isolate small airway epithelial cells from CF and non-CF pigs and established an organotypic culture model. Compared with non-CF large airways, non-CF small airway epithelia cultures had higher [Cl.sup.-] and bicarbonate (HC[O.sup.-.sub.3]) short-circuit currents and higher airway surface liquid (ASL) phi under 5% C[O.sub.2] conditions. CF small airway epithelia were characterized by minimal [Cl.sup.-] and HC[O.sup.-.sub.3] transport and decreased ASL pH, and had impaired bacterial killing compared with non-CF small airways. In addition, CF small airway epithelia had a higher ASL viscosity than non-CF small airways. Thus, the activity of CFTR is higher in the small airways, where it plays a role in alkalinization of ASL, enhancement of antimicrobial activity, and lowering of mucus viscosity. These data provide insight to explain why the small airways are a susceptible site for the bacterial colonization. cystic fibrosis transmembrane conductance regulator doi: 10.1152/ajplung.00422.2015.
Details
- Title: Subtitle
- Electrolyte transport properties in distal small airways from cystic fibrosis pigs with implications for host defense.(Report)(Author abstract)
- Creators
- Mahmoud H. Abou AlaiwaJoseph ZabnerShyam RamachandranXiaopeng LiKelsey SheetsIan M ThornellPhilip H KarpLuis G. Vargas BuonfiglioPeter J TaftDavid K MeyerholzMichael J WelshDavid A StoltzXiao Xiao TangAlejandro P Cornelias
- Resource Type
- Abstract
- Publication Details
- American journal of physiology. Lung cellular and molecular physiology, Vol.310(4), p.L670
- Publisher
- American Physiological Society
- DOI
- 10.1152/ajplung.00422.2015.
- ISSN
- 1040-0605
- eISSN
- 1522-1504
- Language
- English
- Date published
- 04/01/2016
- Description audience
- Academic
- Academic Unit
- Neurosurgery; Roy J. Carver Department of Biomedical Engineering; Pathology; Molecular Physiology and Biophysics; Internal Medicine; Neurology; Pulmonary, Critical Care, and Occupational Medicine; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984201250002771
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