Abstract
Enteral Supplementation with Bovine Lactoferrin or Glutamine Alters Neonatal Iron Status, Intestinal Permeability and Sepsis-related Mortality in a Mouse Model
The Journal of immunology (1950), Vol.212(1_Supplement), pp.476-476_4532
05/01/2024
DOI: 10.4049/jimmunol.212.supp.0476.4532
Abstract
Abstract A murine model of E. coli sepsis results in significant mortality in pups but universal survival in adults despite equivalent immune responses, suggesting that neonatal susceptibility is due to immune-independent factors. Previous data shows that increased intraperitoneal(IP) iron in pups contributes to increased E. coli growth and subsequent mortality. Neonates are known to have decreased intestinal barrier function, so we hypothesized this iron-rich depot was due to a leaky intestinal barrier allowing iron to more freely pass from the intestinal lumen into the IP cavity. To determine if altering intestinal barrier function would influence sepsis-associated mortality, neonates were enterally gavaged with lactoferrin(LF) or glutamine(Gln) daily. LF decreased intestinal permeability when measured by FITC-dextran assay(p<0.0001) and resulted in less IP iron (p<0.0001). LF also resulted in decreased E.coli translocation following E.coli oral gavage sepsis(p=0.0011) and decreased mortality following E.coli IP sepsis(p<0.05). Gln increased intestinal permeability when measured by FITC-dextran assay(p<0.0001) but had no effect on IP iron. Gln increased E.coli translocation(p<0.05) and burden(p<0.05) following E.coli oral gavage sepsis. Gln also increased mortality following E.coli IP sepsis(p<0.05). Overall, early-life supplementation can alter intestinal permeability and iron status, and these alterations influence neonatal E.coli sepsis-associated mortality.
Details
- Title: Subtitle
- Enteral Supplementation with Bovine Lactoferrin or Glutamine Alters Neonatal Iron Status, Intestinal Permeability and Sepsis-related Mortality in a Mouse Model
- Creators
- Kyra Watral - University of IowaJennifer Bermick - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The Journal of immunology (1950), Vol.212(1_Supplement), pp.476-476_4532
- DOI
- 10.4049/jimmunol.212.supp.0476.4532
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 05/01/2024
- Academic Unit
- Stead Family Department of Pediatrics; Neonatology
- Record Identifier
- 9984748258002771
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