Abstract
Evaluating the impact of the CD47 on RBC morphology following sublethal irradiation 2261226
The Journal of immunology (1950), Vol.215(Supplement_1), vkag1411051
08/01/2026
DOI: 10.1093/jimmun/vkag141.1051
Abstract
Introduction
Acute radiation syndrome (ARS) results from exposure to ionizing radiation during medical treatment, nuclear accidents, & space travel. A common side effect of ARS is bone marrow damage & anemia. Healthy red blood cells (RBCs) highly express CD47. A previous study has shown progressive decrease in CD47 expression by RBCs following radiation exposure, suggesting RBC damage & death. Preliminary data from our lab shows Cd47-/- mice are highly susceptible to radiation. Clearance of damaged RBCs is typically mediated by SIRPa expressing phagocytes following decrease in CD47 expression by RBCs. Recent work has revealed RBCs can also undergo programmed inflammatory cell death known as spectosis. To this end, we evaluated RBC death & morphology, as well as anemia following radiation in the absence of CD47.
Methods
We non-lethally irradiated C57BL/6, Cd47-/-, & Cd47-/-Sirpa-/- (DKO) mice. Mice were bled retroorbitally at multiple time points during the recovery period. We evaluated RBC morphology via blood smears, total white blood cells, RBCs, and complete blood counts.
Results
We observed slight anemia in Cd47-/- compared to controls, and even more exaggerated anemia in DKO. We also observed more morphological abnormalities in the absence of CD47 than in controls.
Conclusion
This data suggests that CD47 is required for efficient recovery of the hematopoietic compartment following radiation. This is a potential mechanism mediating susceptibility of Cd47-/- to ionizing radiation.
Details
- Title: Subtitle
- Evaluating the impact of the CD47 on RBC morphology following sublethal irradiation 2261226
- Creators
- Shay Pechter - University of Iowa, Internal MedicinePrajwal Gurung - University of IowaHanna Paton - University of IowaSaurabh Saini - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The Journal of immunology (1950), Vol.215(Supplement_1), vkag1411051
- DOI
- 10.1093/jimmun/vkag141.1051
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- OXFORD UNIV PRESS
- Language
- English
- Date published
- 08/01/2026
- Academic Unit
- Infectious Diseases; Internal Medicine
- Record Identifier
- 9985214919502771
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