Abstract
Expression of Anti‐Aging Gene Klotho in Mouse Blood Vessels
The FASEB journal, Vol.20(4), pp.A729-A729
03/2006
DOI: 10.1096/fasebj.20.4.A729-b
Abstract
Klotho gene was identified in mice with premature aging in which the gene was disrupted. Aorta of those mice manifested undetectable eNOS expression and impaired endothelium‐dependent relaxation. Expression of klotho has not been examined in other blood vessels or compared with aorta, in which expression is low. We examined expression of klotho in blood vessels using real‐time RT‐PCR. Expression of klotho in C57BL/6 mice was low in aorta (7.4±5.1 copies/ng RNA; or 5.4e‐5±2.0e‐5 vs. β‐actin; mean±SE, n=4), carotid artery (2.3±0.6; 2.5e‐5±0.8e‐5), and coronary artery (9.4±7.4; 1.2e‐4±1.0e‐4). Levels were dramatically higher in intracranial vessels (basilar artery and arterioles) (1230±666; 8.2e‐3±4.3e‐3; P<0.05 vs. aorta) than in extracranial arteries. In eNOS−/− mice, klotho expression in basilar artery was as low (1.8±1.4 copies/ng RNA; 4.2e‐5±1.9e‐5 vs. β‐actin; n=3) as in other vessels, suggesting a reciprocal regulation of klotho and eNOS. Because Klotho protein upregulates expression of sod2 (MnSOD) in cell lines and skeletal muscle of klotho‐overexpressing transgenic mice, we determined expression levels of sod1, 2, and 3. In intracranial blood vessels, where klotho expression is highest, expression of sod2 was similar to aorta. This finding suggests that Klotho is not a primary determinant for expression of sod2 in blood vessels. In summary, our findings suggest that intracranial vessels may be an important source for klotho expression, and imply that Klotho may contribute to vasoprotection of cerebral vessels. Expression levels of eNOS may modulate expression and effects of Klotho in cerebral vessels.
Details
- Title: Subtitle
- Expression of Anti‐Aging Gene Klotho in Mouse Blood Vessels
- Creators
- Yi Chu - University of IowaJiro Kitayama - University of IowaShinichiro Iida - University of IowaJordan Miller - University of IowaSu Zhang - University of IowaFrank Faraci - University of IowaDonald D. Heistad - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.20(4), pp.A729-A729
- Publisher
- Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.20.4.A729-b
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Language
- English
- Date published
- 03/2006
- Academic Unit
- Neuroscience and Pharmacology; Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984304740202771
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