Abstract
Genetic Depletion Of Acid-Sensing Ion Channel 3 In Macrophage/Monocytes Prevents Development Of Activity-Induced Muscle Pain
The journal of pain, Vol.24(4 Suppl.), p.32
04/2023
DOI: 10.1016/j.jpain.2023.02.105
Abstract
We developed an animal model of activity-induced muscle pain that combines muscle fatigue with a non-painful, low-dose muscle insult. We previously showed that pharmacological blockade of acid-sensing ion channel 3 (ASIC3) in muscle prevents the activity-induced muscle pain, however, genetic deletion of ASIC3 in primary afferents innervating muscle has no effect on pain. We hypothesized that genetic deletion of ASIC3 on macrophages would prevent activity-induced muscle pain. ASIC3fl/fl mice were crossed with Cx3cr1-Cre mice to generate macrophage/monocyte-specific deletion of ASIC3. To confirm the genetic deletion of ASIC3, expression of ASIC3 was assessed from the peritoneal macrophages using quantitative PCR. Muscle pain was induced by 2, 20μl pH 5.0 injections, 5 days apart, in the gastrocnemius muscle combined with fatiguing muscle contractions in male and female mice. To assess hyperalgesia, muscle withdrawal thresholds (MWT) of the gastrocnemius muscle were measured before and after induction of the model. There was a decrease in ASIC3 mRNA expression in peritoneal macrophages from Cx3cr1CreASIC3fl/fl mice. The decrease in MWT was prevented in Cx3cr1CreASIC3fl/fl mice in both male and female mice, when compared to genetic controls (Cx3cr1CreASIC3+/+)(p<0.05). The current data suggests ASIC3 on macrophages/monocytes mediates the activity-induced muscle pain in male and female mice. This study is supported by the NIH Grant AR073187 and JSPS Fellowship. NIH Grant AR073187 and JSPS Fellowship.
Details
- Title: Subtitle
- Genetic Depletion Of Acid-Sensing Ion Channel 3 In Macrophage/Monocytes Prevents Development Of Activity-Induced Muscle Pain
- Creators
- Kazuhiro HayashiJoseph LesnakAshley N. PlumbLynn A. RasmussenKathleen A. Sluka
- Resource Type
- Abstract
- Publication Details
- The journal of pain, Vol.24(4 Suppl.), p.32
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.jpain.2023.02.105
- ISSN
- 1526-5900
- eISSN
- 1528-8447
- Language
- English
- Date published
- 04/2023
- Academic Unit
- Nursing; Neuroscience and Pharmacology; Physical Therapy and Rehabilitation Science; Iowa Neuroscience Institute
- Record Identifier
- 9984381022902771
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