Abstract
Geometrical deformation of the canine vocal fold induced by formalin fixation
The Journal of the Acoustical Society of America, Vol.109(5_Supplement), pp.2413-2413
05/01/2001
DOI: 10.1121/1.4744527
Abstract
Many existing data on vocal fold geometry are based on anatomical measurements made on histologically prepared vocal fold tissues, commonly by formalin fixation. However, the reliability and validity of these geometrical data are questionable because of the potentially significant tissue deformation associated with histological fixation. As we have shown that reliable geometrical data may be obtained on tissue samples quickly frozen with liquid nitrogen [Tayama et al., Ann. Otol. Rhinol. Laryngol. (in press)], this study attempted to quantify the geometrical deformation of vocal fold tissues fixed with formalin with respect to quick-frozen tissues. Excised canine larynges were divided into two halves along the sagittal plane, one of which was quickly frozen and the other was fixed with formalin. Coronal sections of the frozen halves were thawed and histological slides were prepared from the fixed halves. Measurements of vocal fold geometry (depth and thickness) were made on images of midmembranous coronal sections of the samples. Results showed that significant shrinkage of the vocal fold body (thyroarytenoid muscle) and considerable distortion of the vocal fold cover (free edge) were associated with formalin fixation. These artifacts should be carefully considered when any geometrical data based on histologically fixed tissues are used for laryngeal biomechanical modeling.
Details
- Title: Subtitle
- Geometrical deformation of the canine vocal fold induced by formalin fixation
- Creators
- Miwako Kimura - The University of TokyoNiro Tayama - The University of TokyoRoger W. Chan - Purdue University West LafayetteIngo R. Titze - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The Journal of the Acoustical Society of America, Vol.109(5_Supplement), pp.2413-2413
- DOI
- 10.1121/1.4744527
- ISSN
- 0001-4966
- eISSN
- 1520-8524
- Language
- English
- Date published
- 05/01/2001
- Academic Unit
- School of Music; Communication Sciences and Disorders
- Record Identifier
- 9984719736202771
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