Abstract
HLA Class II Polymorphisms Influence Gut Microbiota Composition and Modulate Disease in Transgenic Mice Model of Multiple Sclerosis
The Journal of immunology (1950), Vol.208(1_Supplement), pp.158-158.15
05/01/2022
DOI: 10.4049/jimmunol.208.Supp.158.15
Abstract
Abstract Multiple sclerosis (MS) is an inflammatory, demyelinating disease of the central nervous system with unknown etiology. The interaction of both genetic and environmental factors plays an important role in MS pathogenesis. Recently, gut microbiota has emerged as a potential environmental factor in MS pathology due to its ability to modulate host immune response. Yet, it is unknown whether genetic factors, such as HLA class II gene(s), can influence gut microbiota which then can modulate MS through regulation of the host immune response. In the present study, we investigated the involvement of gut microbiota in HLA class II genes mediated susceptibility and resistance to MS/experimental autoimmune encephalomyelitis-EAE (an animal model of MS). Previously, we showed that HLA-DR3 transgenic mice lacking endogenous mouse class II genes (AE-KO) were susceptible to proteolipid protein-PLP91–110 induced EAE, whereas AE-KO.HLA-DQ8 transgenic mice were resistant to PLP91–110. Surprisingly, HLA-DR3.DQ8 double transgenic mice showed increased EAE severity compared with HLA-DR3 mice. Distinct microbiota in HLA-DR3, HLA-DQ8, and HLA-DR3.DQ8 transgenic mice compared to AE-KO mice suggested an important role of HLA class-II gene in shaping the gut microbiota composition. We also observed that gut microbiota of HLA-DQ8 mice was more similar to HLA-DR3.DQ8 than HLA-DR3. As the presence of HLA-DQ8 on an HLA-DR3 background increases disease severity, our data suggest that HLA-DQ8 restricted microbiota may contribute to disease severity in HLA-DR3.DQ8 mice. Altogether, our study provides evidence that the HLA-DR and -DQ genes linked to specific gut microbiota may contribute to EAE susceptibility or resistance in a transgenic animal model of MS. The authors acknowledge funding from the National Multiple Sclerosis Society (RG 5138A1/1T), NIAID/NIH (1R01AI137075-01), a Carver Trust Medical Research Initiative Grant, and the University of Iowa Environmental Health Sciences Research Center, NIEHS/NIH (P30 ES005605). SA was supported by the Emory Warner Fellowship, which provides medical students at the Carver College of Medicine the opportunity to take a full year out of their medical school curriculum to work in a laboratory in the University of Iowa Department of Pathology.
Details
- Title: Subtitle
- HLA Class II Polymorphisms Influence Gut Microbiota Composition and Modulate Disease in Transgenic Mice Model of Multiple Sclerosis
- Creators
- Shailesh K ShahiSoham AliPeter LehmanSudeep GhimireNatalya V GusevaAshutosh K Mangalam
- Resource Type
- Abstract
- Publication Details
- The Journal of immunology (1950), Vol.208(1_Supplement), pp.158-158.15
- DOI
- 10.4049/jimmunol.208.Supp.158.15
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 05/01/2022
- Academic Unit
- Pathology; Iowa Neuroscience Institute
- Record Identifier
- 9984355058602771
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