Abstract
Identification of Novel Isoforms of Regulator of G‐Protein Signaling 6 (RGS6)
The FASEB journal, Vol.24(S1), pp.587.2-587.2
04/2010
DOI: 10.1096/fasebj.24.1_supplement.587.2
Abstract
Abstract only RGS6 is a member of the RGS family of proteins that play critical roles in attenuating G protein‐coupled receptor signaling. In addition to a hallmark RGS domain that mediates inactivation of Gi/o subunits, certain RGS6 isoforms possess DEP and GGL domains implicated in their membrane anchoring and binding to Gβ5, respectively. Half of the 36 splice variants of RGS6 we identified in human brain encode DEP domain‐containing long isoforms of RGS6 (RGS6L). In this study, we generated a highly specific antibody against RGS6L isoforms. Using this antibody to evaluate expression of RGS6L in mouse tissues, we identified an immuno‐reactive band corresponding to the predicted molecular weight of RGS6L isoforms in numerous tissues including brain, cerebellum and spinal cord. Moreover, additional unique bands were present in brain, liver and kidney. The size of these other immuno‐reactive proteins could not be accounted for by any known RGS6L transcript. Both the predicted and unique RGS6L immuno‐reactive bands were present in lysates derived from wild‐type but not RGS6 knockout mice, suggesting that the unique bands represent novel isoforms of RGS6L. This is the first evidence that multiple and novel isoforms of RGS6L are present in various mouse tissues. The biological function of the novel isoforms of RGS6L are under investigation. (Supported by NIH GM075033, AHA 0750057Z).
Details
- Title: Subtitle
- Identification of Novel Isoforms of Regulator of G‐Protein Signaling 6 (RGS6)
- Creators
- Jianqi Yang - University of IowaStella‐Rita Ibeawuchi - University of IowaJie Huang - University of IowaAdele Stewart - University of IowaChing‐Kang Jason Chen - Virginia Commonwealth UniversityRory A Fisher - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.24(S1), pp.587.2-587.2
- DOI
- 10.1096/fasebj.24.1_supplement.587.2
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Language
- English
- Date published
- 04/2010
- Academic Unit
- Neuroscience and Pharmacology; Internal Medicine; Iowa Neuroscience Institute
- Record Identifier
- 9984618495702771
Metrics
1 Record Views