Abstract
Impaired vaccine-elicited humoral protection in sepsis surviving hosts 3760
The Journal of immunology (1950), Vol.214(Supplement_1), vkaf2831525
11/01/2025
DOI: 10.1093/jimmun/vkaf283.1525
Abstract
Abstract Description
Sepsis, the life-threatening organ dysfunction stemming from the severe inflammatory response to systemic microbial infection, can affect humans of any age. One consequence of sepsis is the development of prolonged immunoparalysis that contributes to increased susceptibility to infection. With vaccination being an important means for protecting humans from infection throughout their lifetime, it is surprising that there has been no study performed to date assessing vaccine efficacy in sepsis survivors. We found neonatal and adult mice given an intramuscularly delivered inactivated multivalent influenza vaccine 30 days after sepsis induction had reduced total and class switched Ab titers, compared to non-septic controls. The reduced humoral response was due, in part, to impaired CD4 T cell and B cell responses to the vaccine. Reduced Ab titers were similarly seen in post-septic mice given an intranasal live-attenuated influenza vaccine. ELISpot data demonstrating reduced numbers of antibody secreting cells in the spleens of vaccinated septic mice further support the ELISA results. Moreover, mice vaccinated prior to sepsis induction also showed reduced antibody titers. Importantly, vaccinated septic mice had reduced protection to a lethal influenza challenge, demonstrated by increased mortality and viral burden in the lungs. These data collectively show how sepsis can lead to increased susceptibility to pathogens normally neutralized by vaccine-induced immunity.
Funding Sources
Supported by NIH/NIAID R35 GM140881; 5R01 GM139046; 1T32TR004385
Topic Categories
Translational and Interventional Immunology (TI)
Details
- Title: Subtitle
- Impaired vaccine-elicited humoral protection in sepsis surviving hosts 3760
- Creators
- Caleb Y. KimCara Skon-HeggEmma C. KozurekTamara A. KucabaKathryn A. KnoopVladimir P. Badovinac - University of IowaThomas S. Griffith
- Resource Type
- Abstract
- Publication Details
- The Journal of immunology (1950), Vol.214(Supplement_1), vkaf2831525
- DOI
- 10.1093/jimmun/vkaf283.1525
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- Oxford University Press
- Grant note
- NIH/NIAID: R35 GM140881, 5R01GM139046, 1T32TR004385
Supported by NIH/NIAID R35 GM140881; 5R01GM139046; 1T32TR004385
- Alternative title
- IMMUNOLOGY2025™ Abstracts
- Language
- English
- Date published
- 11/01/2025
- Academic Unit
- Pathology
- Record Identifier
- 9985034927702771
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