Abstract
Increased expression of acetaldehyde dehydrogenase in cisplatin-resistant human lung adenocarcinoma A549/DDP cells
Xi bao yu fen zi mian yi xue za zhi, Vol.31(5), pp.625-629
05/2015
PMID: 25940289
Abstract
To investigate the expression and biological significance of acetaldehyde dehydrogenase (ALDH) in cisplatin-resistant human A549/DDP lung adenocarcinoma cell line.
The expressions of biomarkers of cancer stem cells (CSCs) including CD44, CD73, CD90, CD105, epithelial cell adhesion molecule (EpCAM), ATP-binding cassette sub-family G member 2 (ABCG2) and ALDH in human lung adenocarcinoma A549 cells and cisplatin-resistant A549/DDP cells were ascertained by flow cytometry. The proliferation and sensitivity of A549/DDP cells to cisplatin were evaluated by MTT assay before and after ALDH inhibitor diethylaminobenzaldehyde (DEAB) treatment. The differential expression of ALDH subtypes in cells was determined by real-time quantitative PCR, and was further confirmed by a Western blotting.
The frequencies of ABCG2 and ALDH positive cells were higher in cisplatin-resistant A549/DDP cells than those in A549 cells, in particular the expression of ALDH which was 97.7% in A549/DDP cells, and only 4.3% in A549 cells. There
Details
- Title: Subtitle
- Increased expression of acetaldehyde dehydrogenase in cisplatin-resistant human lung adenocarcinoma A549/DDP cells
- Creators
- Jinxi He - Ningxia Medical UniversityXumei Song - Ningxia Medical UniversityLiang Yu - Ningxia Medical UniversityJing Li - Ningxia Medical UniversityZhixiong Qiao - Ningxia Medical UniversityRui Jiu - Ningxia Medical UniversityBo Yu - Ningxia Medical UniversityXiaoming Liu - Ningxia Medical University
- Resource Type
- Abstract
- Publication Details
- Xi bao yu fen zi mian yi xue za zhi, Vol.31(5), pp.625-629
- PMID
- 25940289
- ISSN
- 1007-8738
- Language
- Chinese
- Date published
- 05/2015
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984288627202771
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