Abstract
Induction of BMP4 in Vascular Smooth Muscle Cells by Shear Stress
The FASEB journal, Vol.20(5), pp.A1176-A1177
03/2006
DOI: 10.1096/fasebj.20.5.A1176-b
Abstract
Introduction
Bone morphogenic protein‐4 (BMP4) promotes inflammatory responses and vascular calcification. Smooth muscle cells proliferation and migration occur in the denuded arteries post angioplasty. We assessed whether pulsatile shear stress (PSS) vs. oscillatory shear stress (OSS) regulated BMP2 and BMP4 expression in bovine aortic endothelial cell (BAEC) and vascular smooth muscle cell (VSMC).
Methods
Confluent BAEC and VSMC monolayers were exposed to PSS at a mean shear stress (τave) of 23 dyn.cm−2 and a temporal gradient (∂τ/∂t) at 71 dyn.cm.−2.sec−2; and OSS at τave= 0.02 ± 3 dyn.cm−2 in a dynamic parallel plate flow system for 4 hours. BMP‐mRNA was measured with real‐time RT‐PCR
Results
a) VSMC (Fig. 1): OSS significantly up‐regulated BMP4 by 2.2‐fold and PSS by 1.64‐fold (P<0.05, n=3) in VSMC. OSS significantly down‐regulated BMP2 by 0.32‐fold (P < 0.05), but PSS‐induced down‐regulation was statistically insignificant. Control samples were under static condition.
1
Smooth Muscle cell BMP mRNA expression b) BAEC (Fig. 2): OSS up‐regulated BMP2 by 2‐fold, and BMP4 by 1.5‐fold in BAEC. Similarly, PSS induced BMP2 and BMP4 expression by 1.6‐ and 1.4‐fold, respectively. However, these differences were statistically insignificant.
2
Endothelial BMP mRNA expression
Discussion
OSS was a stronger inducer of BMP4 expression in VSMC than PSS. The findings suggest that shear stress mediated increases in BMP4 expression may contribute to inflammation in the denuded regions of stented arteries.
Details
- Title: Subtitle
- Induction of BMP4 in Vascular Smooth Muscle Cells by Shear Stress
- Creators
- Mahsa Rouhanizadeh - University of Southern CaliforniaTiantian C Lin - University of Southern CaliforniaJordan D Miller - University of IowaDonald Heistad - University of IowaTzung K Hsiai - University of Southern California
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.20(5), pp.A1176-A1177
- Publisher
- Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.20.5.A1176-b
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 2
- Language
- English
- Date published
- 03/2006
- Academic Unit
- Cardiovascular Medicine; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984303995302771
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