Abstract
Ischemic Factors Precipitate a Shift in Immune Cell Populations at the Site of Cerebrovascular Blockage
The Journal of immunology (1950), Vol.204(1_Supplement), pp.64-64.17
05/01/2020
DOI: 10.4049/jimmunol.204.Supp.64.17
Abstract
Abstract The incidence of stroke has risen over the past decade and will continue to be one of the leading causes of adult disability and death worldwide. Reperfusion therapy or endovascular thrombectomy (EVT) for large vessel occlusion (LVO) remains the only proven stroke therapy during the acute phase. But a select set of patients still exhibit poor recovery of neurological function, despite active cortical reorganization. The mechanisms that underlie this neurological dysfunction is still poorly understood. Subsequent to ischemic injury, a massive inflammatory response is seen for days, which exacerbates neuropathology in stroke models. Within the draining lymph nodes of ischemic patients, innate immune cells are actively phagocytizing brain antigen and presenting them to adaptive immune cells. The focus of this project is to determine if early changes in immune cells near the site of occlusion can be used as predictors of long-term neurofunctional decline. Acute anterior circulation LVO patients treated with EVT will be enrolled in this study. At the time of thrombectomy, blood was collected proximal and distal to the thrombus prior to recanalization. As a control, blood was also collected from the femoral artery. Deep immune profiling of innate and adaptive immune cells using 13-parameter flow cytometry was performed revealing a specific increase in post-thrombus CD4 T-cells, as compared to pre-thrombus. Cytokine analysis revealed a decrease in pre- and post-thrombus IFN-γ and IL1α. Induction of in vitro hypoxia in healthy peripheral blood mononuclear cells revealed a similar reduction in IFN-γ. We hypothesize that ischemia precipitates a shift in immune cells at the site of cerebrovascular blockage that modulates stroke recovery.
Details
- Title: Subtitle
- Ischemic Factors Precipitate a Shift in Immune Cell Populations at the Site of Cerebrovascular Blockage
- Creators
- Sterling B OrtegaCynthia B ZevallosDarko Q OrozcoDaniel TranelNitin J. KarandikarSantiago Ortega-Gutierrez
- Resource Type
- Abstract
- Publication Details
- The Journal of immunology (1950), Vol.204(1_Supplement), pp.64-64.17
- DOI
- 10.4049/jimmunol.204.Supp.64.17
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 05/01/2020
- Academic Unit
- Pathology; Radiology; Neurology; Psychological and Brain Sciences; Iowa Neuroscience Institute; Neurosurgery
- Record Identifier
- 9984354512002771
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