Logo image
Back
LB1036 Spatial proteomics of immune checkpoints in discoid lupus and lichen planus
Abstract   Peer reviewed

LB1036 Spatial proteomics of immune checkpoints in discoid lupus and lichen planus

U. Yildiz Altay, R. Breidbart, M. Kidacki, A. Jaiswal, C. Cho and M.D. Vesely
Journal of investigative dermatology, Vol.145(8 Suppl), pp.S180-S180
08/2025
DOI: 10.1016/j.jid.2025.06.1307

View Online

Abstract

Interface dermatoses a histopathological pattern observed in several autoimmune connective tissue diseases, including discoid lupus erythematosus (DLE) and lichen planus (LP). Despite distinct clinical presentations, both diseases share histologic features suggestive of common underlying pathogenic mechanisms. However, their immunoregulatory landscapes remain unclear. This study employs spatial proteomics to characterize immune inhibitory receptor expression in DLE and LP, revealing disease-specific and overlapping immune checkpoint pathways. We analyzed archival DLE and LP skin biopsies using the NanoString GeoMx Immuno-Oncology protein panel. Heatmap and UMAP clustering demonstrated distinct protein expression profiles between DLE and LP. Cross-segmentation analysis of keratinocytes (PanCK+), CD4+, and CD8+ T cells identified differential immune checkpoint and T cell activation expression. In DLE, immune modulation was compartmentalized, with keratinocytes expressing PD-L2, CD44, and CD127 compared to CD4+ and CD8 T cells. Additionally, CD4+T cells in DLE expressed greater ICOS, CTLA4, OX40L, CD40, 4-1BB, and PD-1 compared to CD8+ T cells. In contrast to DLE, CD4+ and CD8+ T cells within LP tissues expressed VISTA, PD-L1, and STING, whereas keratinocytes express OX40L, CD127, CD44 and ARG1. These findings underscore key immunoregulatory differences in interface dermatoses and suggest potential therapeutic strategies, including immune checkpoint agonists, for targeted intervention in autoimmune dermatoses.

Details

Metrics

3 Record Views
Logo image