Abstract
M‐CURRENT IN NODOSE SENSORY NEURONS MEDIATES THE DEPOLARIZING EFFECT OF PROSTACYCLIN
The FASEB journal, Vol.21(6), pp.A1407-A1407
2007
DOI: 10.1096/fasebj.21.6.A1407-a
Abstract
We have shown that carbacyclin (cPGI), a stable analog of prostacyclin, depolarizes baroreceptor nodose neurons and increases their excitability. The latter action is mediated by protein kinase A dependent inhibition of Maxi‐K channels. In this study we asked whether the depolarizing effect of cPGI is mediated by inhibition of M‐current. Linopirdine (30 μM), a selective direct inhibitor of M current, depolarized isolated nodose neurons by 17.6±5.8 mV (n=5, P<0.05) and decreased their conductance by 7.4±3.1 nS (P<0.05). cPGI (10 μM) did not depolarize the neurons in the presence of linopirdine suggesting that inhibition of M‐current mediated the cPGI‐induced depolarization. To verify this finding we used a patch clamp protocol for M‐current detection. The neurons were voltage clamped at −20 mV and hyperpolarized with 500 msec pulses to −60 mV every 4 seconds. During the hyperpolarizing pulses, the current increased by 20.3±7.9 pA (n=8) over the period from 50 to 500 msec. In contrast, after linopirdine or after cPGI the current decreased by 20.2±7.8 (n=4) and 16.3±7.6 (n=4) pA, respectively. Application of cPGI in the presence of linopirdine did not alter the change in the current during the hyperpolarizing pulses. We conclude that linopirdine sensitive M‐current is present in nodose sensory neurons and its inhibition mediates cPGI‐induced depolarization (HL14388)
Details
- Title: Subtitle
- M‐CURRENT IN NODOSE SENSORY NEURONS MEDIATES THE DEPOLARIZING EFFECT OF PROSTACYCLIN
- Creators
- Vladislav Snitsarev - University of IowaCarol A Whiteis - University of IowaMark W Chapleau - VA Medical CenterFrancois M Abboud - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.21(6), pp.A1407-A1407
- Publisher
- The Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.21.6.A1407-a
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Language
- English
- Date published
- 2007
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine; Molecular Physiology and Biophysics
- Record Identifier
- 9984303000602771
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