Abstract
Maternal low protein diet or dexamethasone administration impairs endothelial‐dependent reactivity and glucose tolerance in a murine model of fetal programming
The FASEB journal, Vol.20(4), pp.A758-A758
03/2006
DOI: 10.1096/fasebj.20.4.A758-a
Abstract
Background
Low birth weight is a risk factor for hypertension and diabetes. In rats, maternal low protein diet increases fetal glucocorticoid exposure and programs cardiovascular and endocrine dysfunction in adulthood. Development of an analogous murine model would facilitate genetic manipulation for etiologic definition.
Methods
Iso‐caloric low protein diet (LP, 9% casein) was provided to dams from E0 to E19. Additional dams received a normal protein diet (18% casein) without (NP) or with either dexamethasone (NP‐Dex, 0.1 mg/kg/d sc) or normal saline (NP‐NS) from E10 to E18 (n = 10 per group).
Results
Offspring of dams given LP weighed less at 10 days than NP offspring (LP 5.1 ± 0.1, NP 6.1 ± 0.2 g). At 6 months, all four groups had similar tail cuff blood pressures, but the offspring of both LP and NP‐Dex exposed dams displayed (1) impaired endothelial‐dependent vasodilatation (tone remaining after 1 microM/L acetylcholine: LP 42 ± 6, NP 25 ± 4, NP‐Dex 44 ± 10, NP‐NS 30 ± 7 %) and (2) reduced glucose clearance rates (LP 1.8 ± 0.1, NP 2.8 ± 0.2, NP‐Dex 2.4 ± 0.3, NP‐NS 3.0 ± 0.3 mg/dL/min).
Conclusion
Maternal LP impairs (1) perinatal growth, (2) endothelial‐dependent vascular reactivity, and (3) glucose clearance. This model of fetal programming is replicated by late gestation dexamethasone administration (NP‐Dex group) but without growth restriction, suggesting exposure of the murine fetus to maternally‐derived glucocorticoids mediates maternal LP‐induced fetal programming in a species amenable to genetic manipulation.
Supported by a grant from the Children’s Miracle Network
Details
- Title: Subtitle
- Maternal low protein diet or dexamethasone administration impairs endothelial‐dependent reactivity and glucose tolerance in a murine model of fetal programming
- Creators
- Robert Dean Roghair - University of IowaJeffrey L Segar - University of IowaThomas D Scholz - University of IowaFred S Lamb - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.20(4), pp.A758-A758
- Publisher
- Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.20.4.A758-a
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Grant note
- Children’s Miracle Network
- Language
- English
- Date published
- 03/2006
- Academic Unit
- Child and Community Health; Fraternal Order of Eagles Diabetes Research Center; Stead Family Department of Pediatrics; Cardiology; Neonatology
- Record Identifier
- 9984354389902771
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