Abstract
Murine chorioamnionitis alters the neonatal and infant immune response to respiratory syncytial virus
The Journal of immunology (1950), Vol.212(1_Supplement), pp.1144-1144_5151
05/01/2024
DOI: 10.4049/jimmunol.212.supp.1144.5151
Abstract
Abstract Chorioamnionitis is infection and/or inflammation of the fetal membranes and placenta during pregnancy and is a risk factor for neonatal bronchopulmonary dysplasia (BPD) and childhood asthma and wheezing. BPD and childhood asthma are associated with more severe infection with respiratory syncytial virus (RSV). Despite this, it is unknown whether chorioamnionitis alters the neonatal or infant immune response to RSV. To investigate this, we used a murine model of chorioamnionitis and infected pups with RSV on postnatal days 3-5 (neonate) or postnatal days 14-16 (infant). 5-days post-infection, mRNA was isolated from lungs and analyzed via qPCR for expression of MUC5AC, GOB5, IL1B, IL5, IL6, IL13, IL17A, IFNB, IFNG, RSV-N, RSV-F, RSV-G, and GAPDH. We hypothesized that murine chorioamnionitis-exposed neonatal and infant offspring would display increased severity of RSV infection with increased lung mRNA expression of pro-inflammatory cytokines and a higher viral burden. Our neonatal results displayed significantly lower relative mRNA expression of IL1B in chorioamnionitis-exposed pups (p=0.0035, Mann-Whitney test). Our infancy results displayed increased expression of RSV-N (p=0.0288), MUC5AC (p=0.0594), and GOB5 (p=0.0510), and decreased expression of IFNB (p=0.0101) in chorioamnionitis-exposed pups. In conclusion, murine chorioamnionitis alters both the neonatal and infant immune response to RSV.
Details
- Title: Subtitle
- Murine chorioamnionitis alters the neonatal and infant immune response to respiratory syncytial virus
- Creators
- Jessica Knobbe - University of IowaJennifer Bermick - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The Journal of immunology (1950), Vol.212(1_Supplement), pp.1144-1144_5151
- DOI
- 10.4049/jimmunol.212.supp.1144.5151
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 05/01/2024
- Academic Unit
- Stead Family Department of Pediatrics; Neonatology
- Record Identifier
- 9984748260202771
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