Abstract
NOS inhibition prevents AMPK induction of GLUT4, citrate synthase and F1ATP synthase mRNA in L6 myotubes
The FASEB journal, Vol.20(5), pp.A820-A820
03/2006
DOI: 10.1096/fasebj.20.5.A820
Abstract
Recently it was shown that nitric oxide (NO) donors can induce mitochondrial biogenesis in different cell types. Endurance exercise induces mitochondrial and GLUT4 biogenesis in skeletal muscle in a process related to AMPâdependent protein kinase (AMPK) phosphorylation and activation. AMPK phosphorylates and activates both eNOS and nNOS. Therefore, we hypothesized that NOS inhibition would prevent AMPKâinduced upregulation in GLUT4, Citrate Synthase (CS) and F1 ATP Synthase mRNA in L6 myotubes. Myoblasts were cultured in growth medium (10% FBS) until reaching 80% confluence, then switched to differentiation medium (2%HoS) until myotubes were 80% confluent. At this point, myotubes were exposed to treatment medium (10%HoS + 5%FBS) for 16 h, with or without the AMPK activator AICARâmonophosphate (5âaminoimidazoleâ4âcarboxamide riboside monophosphate, 1mM) or AICARâmonophosphate + the NOS inhibitors, LâNAME (N(G)âLânitroâarginine methyl ester, 100ÎŒM) or TRIM (1â(2âtrifluoromethylâphenyl)âimidazole, 100ÎŒM).
RESULTS
AICARâmonophosphate induced significant increases in the mRNA levels of the three genes evaluated (n=5â6/group; P<0.01), whereas coâtreatment with LâNAME or TRIM prevented this effect. Incubation of myotubes for 16h with low concentrations of the nitric oxide donor, SNAP (1 and 10ÎŒM), induced increases in both GLUT4 and CS mRNA. Treatment with the cell permeable cGMP analog, 8âBrâcGMP (1 and 2mM), also increased GLUT4 and CS mRNA levels. These findings suggest that nitric oxide plays a role in the AMPKâinduced upregulation of GLUT4, Citrate Synthase and F1 ATP Synthase mRNA, and that NO and cGMP are sufficient to increase GLUT4 and Citrate Synthase transcript levels in skeletal muscle.
Funded by AHA Grant #0530196N.
Details
- Title: Subtitle
- NOS inhibition prevents AMPK induction of GLUT4, citrate synthase and F1ATP synthase mRNA in L6 myotubes
- Creators
- Vitor A Lira - University of FloridaQuinlyn A. Soltow - University of FloridaJodi H. D. Long - University of FloridaJenna L. Betters - University of FloridaJeff E. Sellman - University of FloridaDavid S. Criswell - University of Florida
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.20(5), pp.A820-A820
- Publisher
- Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.20.5.A820
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Grant note
- AHA (#0530196N)
- Language
- English
- Date published
- 03/2006
- Academic Unit
- Health and Human Physiology
- Record Identifier
- 9984267139302771
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