O-035 Hyperacute inflammatory profile in patients with acute ischemic stroke (AIS)

M Farooqui; C Zevallos; D Quispe-Orozco; A Dajles; A Mendez Ruiz; D Tranel; N Karandikar; S Ortega; S Ortega-Gutierrez

doi:10.1136/neurintsurg-2021-SNIS.35

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O-035 Hyperacute inflammatory profile in patients with acute ischemic stroke (AIS)

M Farooqui, C Zevallos, D Quispe-Orozco, A Dajles, A Mendez Ruiz, D Tranel, N Karandikar, S Ortega and S Ortega-Gutierrez

Journal of neurointerventional surgery, Vol.13(Suppl 1), pp.A24-A24

08/2021

DOI: 10.1136/neurintsurg-2021-SNIS.35

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Abstract

IntroductionInflammation is an important mechanism of ischemic brain injury. It is characterized by inflammatory mediators and molecules including cytokines and interleukins. This inflammatory mechanism and its interaction among these Ischemic Stroke (IS) patients is still ambiguous. The aim of this investigation is to elucidate and characterize the early inflammatory response at the site of occlusion among these IS patients.MethodsLarge vessel occlusion acute ischemic stroke (LVO-AIS) patients eligible for Mechanical Thrombectomy (MT) were recruited within 24 hours from their symptom onset. Blood samples were collected proximal and distal to the occlusion site during the procedure. Control samples were collected from the femoral artery and median cubital vein. 20-Plex assay and ELISA was used for the analysis of cytokines and chemokines. Graph-pad prism and R-software was used for evaluating the differences among the molecules across the site of occlusion and control.ResultsA total of 19 (male: 13 and female: 6) patients were included. Cytokine quantification observed a significant increase in MMP-9 and IFN-g proximal to the occlusion, whereas, there was a decrease in IL-2, IL-4, IL-5, IL-6, IL-7, IL-15, IL-17, GM-CSF, TNF-α, IP-10, VEGF, MIP-1a, and MIP-1b distal to the clot. The levels of IL-8, MCP-1, and MIG were comparable across the sites.ConclusionOur results characterized the local environment and immediate inflammatory molecules, within few hours of the ischemic brain injury. These observations indicate the evidence of initial activation of inflammatory response. This will help better understand the molecular patho-physiology and identify molecular biomarkers of ischemic stroke progression and subsequent modulating therapeutic interventions.Disclosures M. Farooqui: None. C. Zevallos: None. D. Quispe-Orozco: None. A. Dajles: None. A. Mendez Ruiz: None. D. Tranel: None. N. Karandikar: None. S. Ortega: None. S. Ortega-Gutierrez: None.

Details

Title: Subtitle: O-035 Hyperacute inflammatory profile in patients with acute ischemic stroke (AIS)
Creators: M Farooqui - University of Iowa
C Zevallos - University of Iowa
D Quispe-Orozco - University of Iowa
A Dajles - University of Iowa Hospitals and Clinics, Iowa City, IA
A Mendez Ruiz - University of Iowa
D Tranel - University of Iowa
N Karandikar - University of Iowa
S Ortega - University of Iowa
S Ortega-Gutierrez - University of Iowa
Resource Type: Abstract
Publication Details: Journal of neurointerventional surgery, Vol.13(Suppl 1), pp.A24-A24
DOI: 10.1136/neurintsurg-2021-SNIS.35
ISSN: 1759-8478
eISSN: 1759-8486
Language: English
Date published: 08/2021
Academic Unit: Neurology; Radiology; Psychological and Brain Sciences; Pathology; Iowa Neuroscience Institute; Neurosurgery
Record Identifier: 9984303554702771

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