Abstract
Oxidative stress after intracranial hemorrhage
The FASEB journal, Vol.21(5), pp.A396-A396
04/2007
DOI: 10.1096/fasebj.21.5.A396-c
Abstract
Mechanisms of brain injury after intracranial hemorrhage (ICH) are not clear. We tested the hypothesis that extravascular blood activates NADPH oxidase and increases superoxide in a mouse model of ICH. Four μl of autologous blood or artificial cerebrospinal fluid (aCSF) were injected into the striatum of anesthetized C57BL/6 mice. Twenty hours later, superoxide levels were measured in homogenized brain. Superoxide levels, using the tiron‐inhibitable fraction of lucigenin‐enhanced chemiluminescence, were increased following blood injection (blood 0.15±0.04 vs aCSF 0.05±0.01 RLU/sec/μg protein, p<0.05) (mean±SE). Using dihydroethidium in brain slices, superoxide was increased in brain parenchyma around the hematoma. NADPH oxidase activity in brain homogenates was greater in the blood injected hemisphere (blood 22±2 vs aCSF 8±1 RLU/sec/μg protein, p<0.001) (mean±SE). Quantitative real‐time PCR demonstrated no change in expression of the catalytic subunits of the NADPH oxidase (Nox1, Nox2, and Nox4). Diphenyliodonium, an inhibitor of NADPH oxidase activity, markedly reduced superoxide levels in both blood and aCSF injected brain. Thus, increases in superoxide may be due to increased activity, but not altered expression, of NADPH oxidese. Collectively, these data suggest that extravascular blood increases superoxide perhaps through activation of NADPH oxidase.
Details
- Title: Subtitle
- Oxidative stress after intracranial hemorrhage
- Creators
- Yoshinobu Wakisaka - University of IowaJordan D Miller - University of IowaDonald D Lund - United States Department of Veterans AffairsYi Chu - University of IowaGary L Baumbach - University of IowaFrank M Faraci - University of IowaDonald D Heistad - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.21(5), pp.A396-A396
- Publisher
- Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.21.5.A396-c
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Language
- English
- Date published
- 04/2007
- Academic Unit
- Pathology; Neuroscience and Pharmacology; Fraternal Order of Eagles Diabetes Research Center; Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984304003902771
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