P1-019 An active zone scaffolding protein functionally associates with presynaptic calcium channels

Takafumi Miki; Shigeki Kiyonaka; Mio Nonaka; Yoshitsugu Uriu; Minoru Wakamori; Emiko Mori; Yuji Hara; Michel De Waard; Makoto Itakura; Masami Takahashi; Haruhiko Bito; Kevin Campbell; Yasuo Mori

doi:10.14849/psjproc.2007.0_224_3

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P1-019 An active zone scaffolding protein functionally associates with presynaptic calcium channels

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P1-019 An active zone scaffolding protein functionally associates with presynaptic calcium channels

Takafumi Miki, Shigeki Kiyonaka, Mio Nonaka, Yoshitsugu Uriu, Minoru Wakamori, Emiko Mori, Yuji Hara, Michel De Waard, Makoto Itakura, Masami Takahashi, …

Proceedings of Annual Meeting of the Physiological Society of Japan, Vol.57(suppl.2), pp.S224-S224

2007

DOI: 10.14849/psjproc.2007.0_224_3

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Abstract

The spacing between voltage-dependent calcium channels (VDCCs) and vesicles at active zones is thought to influence the dynamic properties of synaptic transmission. However, molecular determinants that maintain or regulate synaptic vesicles and VDCCs within a physiologically appropriate distance have been elusive. Here, we demonstrate a novel molecular interaction between an active zone protein and VDCCs, that controls neurotransmitter release. GST-pull down and immunoprecipitation assay showed that the active zone protein associated with VDCC β-subunits via its C-terminus. In neuroendocrine PC12 cells, acetylcholine release was significantly potentiated by the full-length, N-terminus, and C-terminus constructs, but the proportion of membrane-docked vesicles was enhanced only by the full-length. Thus, the active zone protein associated with VDCC β-subunits near the active zone supports neurotransmitter release via anchoring of neurotransmitter-containing vesicles in the vicinity of VDCCs.

Details

Title: Subtitle: P1-019 An active zone scaffolding protein functionally associates with presynaptic calcium channels
Creators: Takafumi Miki
Shigeki Kiyonaka
Mio Nonaka
Yoshitsugu Uriu
Minoru Wakamori
Emiko Mori
Yuji Hara - University of Iowa, Molecular Physiology and Biophysics
Michel De Waard
Makoto Itakura
Masami Takahashi
Haruhiko Bito
Kevin Campbell - University of Iowa, Molecular Physiology and Biophysics
Yasuo Mori
Resource Type: Abstract
Publication Details: Proceedings of Annual Meeting of the Physiological Society of Japan, Vol.57(suppl.2), pp.S224-S224
Publisher: The Japanese Pharmacological Society
DOI: 10.14849/psjproc.2007.0_224_3
ISSN: 1347-8613
Language: Japanese
Date published: 2007
Academic Unit: Molecular Physiology and Biophysics; Neurology; Iowa Neuroscience Institute
Record Identifier: 9984068349202771

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