Pitx2 controls dental stem cell proliferation and differentiation by regulating Cyclin D2 and Irx1

Wenjie Yu; Zhao Sun; Steven Eliason; Brad A. Amendt

doi:10.1096/fasebj.31.1_supplement.743.10

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Pitx2 controls dental stem cell proliferation and differentiation by regulating Cyclin D2 and Irx1

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Pitx2 controls dental stem cell proliferation and differentiation by regulating Cyclin D2 and Irx1

Wenjie Yu, Zhao Sun, Steven Eliason and Brad A. Amendt

The FASEB journal, Vol.31(S1)

04/2017

DOI: 10.1096/fasebj.31.1_supplement.743.10

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https://doi.org/10.1096/fasebj.31.1_supplement.743.10View

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Abstract

Abstract only Pituitary homeobox 2 ( PITX2 ), the gene responsible for Axenfeld‐Rieger syndrome (ARS), plays a crucial role in odontogenesis. ARS individuals has dental agenesis and enamel hypoplasia. Although the function of Pitx2 in tooth development is well established, detailed cellular and molecular mechanisms of Pitx2 regulation remain unclear. Here, we conditionally ablated Pitx2 in the dental epithelium using the Krt14‐Cre ( K14 Cre Pitx2 f/f ), which resulted in arrested tooth development at the bud stage. BrdU incorporation analysis showed that cell proliferation is decreased in the K14 Cre Pitx2 f/f incisors. In addition, we found that Sox9+ cells are reduced in the dental epithelium in K14 Cre Pitx2 f/f teeth, indicating that cell differentiation is down‐regulated. We have identified Iroquois 1 (Irx1) as a new homobox transcription factor expressed in early dental development. Mechanistically, Pitx2 regulates the expression of Cyclin D2 and Irx1 to promote early tooth formation. In conclusion, we have uncovered a new Pitx2 dependent regulatory network during early tooth development. Support or Funding Information College of dentistry, the University of Iowa

Details

Title: Subtitle: Pitx2 controls dental stem cell proliferation and differentiation by regulating Cyclin D2 and Irx1
Creators: Wenjie Yu - University of Iowa
Zhao Sun - University of Iowa
Steven Eliason - University of Iowa
Brad A. Amendt - University of Iowa
Resource Type: Abstract
Publication Details: The FASEB journal, Vol.31(S1)
DOI: 10.1096/fasebj.31.1_supplement.743.10
ISSN: 0892-6638
eISSN: 1530-6860
Language: English
Date published: 04/2017
Academic Unit: Orthodontics; Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Dental Research; Internal Medicine
Record Identifier: 9984629021302771

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