Abstract
Pulmonary hypertension is present in a rodent model of bronchopulmonary dysplasia at 21 days but not 6 months of age (715.1)
The FASEB journal, Vol.28(S1), p.n/a
04/2014
DOI: 10.1096/fasebj.28.1_supplement.715.1
Abstract
In preterm infants, mechanical ventilation with supplemental O2 can cause bronchopulmonary dysplasia (BPD), characterized by dysmorphic pulmonary vasculature and simplified alveoli. Although vascular cross‐sectional area is diminished, contributing to right ventricular hypertension (RVH), it is unknown whether this improves with age. We hypothesized that RVH would continue into adulthood in a rat model of BPD. Rats were exposed to 21% or 95% O2 from birth to postnatal day 8. At 21 days or 6 months of age, a fluid‐filled catheter was placed in the RV to measure RV pressure and inject 2x108 4μm fluorescent spheres. At the conclusion of the experiment, lungs were harvested, air dried, and imaged using laser scanning confocal microscopy to investigate capillary density. At 21 days, rats exposed to 95% O2 demonstrated RVH (31±5 mmHg), which was absent in both 21 day controls (16±2 mmHg, p=0.028) and 6 month old rats exposed to 95% O2 (20±10 mmHg, p=0.014). Interestingly, the density and distribution of spheres was not different between 6 month old rats exposed to 95% or 21%, suggesting that the normalization of right heart pressure by 6 months is the result of improved alveolar capillary density. Our findings indicate that children and infants with BPD may be at high risk for RVH, but that this resolves with age.
Grant Funding Source: Supported by NIH 5R01HL115061
Details
- Title: Subtitle
- Pulmonary hypertension is present in a rodent model of bronchopulmonary dysplasia at 21 days but not 6 months of age (715.1)
- Creators
- Aaron Kleinertz - Pediatrics University of WisconsinRobert Conhaim - Surgery University of WisconsinTaylor Shuster - Pediatrics University of WisconsinSidney Mahan - Pediatrics University of WisconsinMarlowe Eldridge - Pediatrics University of WisconsinMelissa Bates - Pediatrics University of Wisconsin
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.28(S1), p.n/a
- Publisher
- The Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.28.1_supplement.715.1
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Grant note
- NIH (5R01HL115061)
- Language
- English
- Date published
- 04/2014
- Academic Unit
- Internal Medicine; Health and Human Physiology; Stead Family Department of Pediatrics; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984288738002771
Metrics
13 Record Views