Abstract
Reduced Endothelium‐Dependent Dilation with Aging in Humans is Associated with Endothelial Oxidative Stress and Enhanced Expression of NADPH Oxidase
The FASEB journal, Vol.21(6), pp.A1372-A1373
2007
DOI: 10.1096/fasebj.21.6.A1372-d
Abstract
In rodents, reductions in endothelium‐dependent dilation (EDD) with aging are associated with the development of vascular oxidative stress. It is unknown if oxidative stress develops with aging in the vascular endothelium of humans and if it is related to reductions in EDD. To determine this, young (Y: 24 ± 1 yr; n = 41) and older (O: 63 ± 1 yr; n = 45) healthy sedentary men were studied. EDD (brachial artery flow‐mediated dilation, FMD) was 46% lower in the older vs. young men (4.3 ± 0.3 vs. 8.0 ± 0.3%, P < 0.01). Endothelial nitrotyrosine (NT) protein expression (quantitative immunofluorescence) was higher in arterial (1.20 ± 0.13 vs. 0.39 ± 0.04 NT intensity/HUVEC intensity, P < 0.01) and venous (0.55 ± 0.07 vs. 0.34 ± 0.03, P < 0.05) endothelial cells (EC) of the older vs. young men. NADPH oxidase p47phox EC protein expression was higher in older subjects (0.67 ± 0.05 vs. 0.56 ± 0.05 NADPH oxidase p47phox intensity/HUVEC intensity, P < 0.05), whereas xanthine oxidase was not different. EC cytosolic and mitochondrial superoxide dismutase expression did not differ. In the overall group, FMD was inversely related to NT expression in arterial EC (r = − 0.63, P < 0.01). These results indicate that oxidative stress develops in the vascular endothelium with aging in healthy sedentary men and is related to reductions in EDD. Increased expression of NADPH oxidase may contribute to endothelial oxidative stress with aging in humans.
Supported by NIH AG013038, AG006537, AG022241, RR00051, and AG00279
Details
- Title: Subtitle
- Reduced Endothelium‐Dependent Dilation with Aging in Humans is Associated with Endothelial Oxidative Stress and Enhanced Expression of NADPH Oxidase
- Creators
- Anthony J Donato - Integrative PhysiologyUniversity of Colorado at Boulder354UCBBoulderCO80309‐0354Adam Levy - Integrative PhysiologyUniversity of Colorado at Boulder354UCBBoulderCO80309‐0354Iratxe Eskurza - Integrative PhysiologyUniversity of Colorado at Boulder354UCBBoulderCO80309‐0354Gary L Pierce - Integrative PhysiologyUniversity of Colorado at Boulder354UCBBoulderCO80309‐0354Annemarie E Silver - Integrative PhysiologyUniversity of Colorado at Boulder354UCBBoulderCO80309‐0354Douglas R Seals - Integrative PhysiologyUniversity of Colorado at Boulder354UCBBoulderCO80309‐0354
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.21(6), pp.A1372-A1373
- Publisher
- The Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.21.6.A1372-d
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Grant note
- NIH (AG013038, AG006537, AG022241, RR00051, and AG00279)
- Language
- English
- Date published
- 2007
- Academic Unit
- Health and Human Physiology; Internal Medicine
- Record Identifier
- 9984267247702771
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