Abstract
Renal Denervation Attenuates Nicotine‐Induced Increase in Thiazide‐Sensitive Na+/Cl− Cotransporter in the Young Pre‐Hypertensive Spontaneously Hypertensive Rat
The FASEB journal, Vol.32(S1), pp.885.20-885.20
04/2018
DOI: 10.1096/fasebj.2018.32.1_supplement.885.20
Abstract
Systemic infusion of nicotine induces renal inflammation and the development of premature hypertension in young spontaneously hypertensive rats (SHR). Renal denervation (RDN) prevents this nicotine‐mediated hypertension and attenuates renal inflammation. We hypothesized that these findings were related to alterations in renal sodium handling. Following bilateral RDN or sham surgery in young (3–5 week old SHR), we continuously infused saline or nicotine bitartrate (15mg/kg/day) subcutaneously for 2 weeks. Urinary sodium was assessed via spectrophotometric analysis on spot urine samples obtained at the end of the infusion. Renal homogenates were assessed by Western blot for the presence of the epithelial sodium channel subunits (ENaC‐alpha, ‐beta, and ‐gamma), sodium‐potassium‐chloride (NKCC2), and thiazide‐sensitive sodium‐chloride co‐transporter (NCC). Systemic infusion of nicotine induced significant sodium retention, compared to saline, in Sham treated animals, as measured by a significant decrease in urinary sodium (from 252±55 mM to 137±26, p<0.05). During nicotine infusion in animals with RDN, there was a trend towards lesser sodium retention and a higher urinary sodium (192±46 mM, p=0.09). Western blot demonstrated that nicotine infusion in Sham treated animals induced a 1.7 fold increase in renal NCC expression, compared to saline (p<0.05). However, there was no nicotine effect on the expression of the three ENaC subunits or NKCC2 (p>0.05). RDN prevented the increase in renal NCC expression in response to nicotine infusion (p<0.05). We conclude that reduced renal sodium retention and decreased expression of NCC may contribute to the prevention of nicotine‐mediated hypertension and renal inflammation by RDN.
Support or Funding Information
National Institutes of Health (NIH K08 HL119588, PPG HL14388)
Spot urinary sodium following Sham surgery or RDN followed by 2 week infusion of subcutaneous saline or nicotine. Urine samples were collected at the end of infusion.
Renal Thiazide‐Sensitive Na+/Cl− Cotransporter (NCC) Expression Following Renal Denervation and 2‐week Subcutaneous Infusion of Saline or Nicotine.
This is from the Experimental Biology 2018 Meeting. There is no full text article associated with this published in The FASEB Journal.
Details
- Title: Subtitle
- Renal Denervation Attenuates Nicotine‐Induced Increase in Thiazide‐Sensitive Na+/Cl− Cotransporter in the Young Pre‐Hypertensive Spontaneously Hypertensive Rat
- Creators
- Nandita Raikwar - University of IowaAlan Ryan - University of IowaPeter M. Snyder - University of IowaFrancois Abboud - University of IowaSailesh Harwani - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.32(S1), pp.885.20-885.20
- Publisher
- The Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.2018.32.1_supplement.885.20
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Grant note
- National Institutes of Health (NIH K08 HL119588; PPG HL14388)
- Language
- English
- Date published
- 04/2018
- Academic Unit
- Molecular Physiology and Biophysics; Medicine Administration; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984302998202771
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