Abstract
Role of angiotensin in endothelial dysfunction after lipopolysaccharide in mice
The FASEB journal, Vol.20(5), pp.A1164-A1164
03/2006
DOI: 10.1096/fasebj.20.5.A1164-c
Abstract
Endotoxin (or lipopolysaccharide, LPS) increases levels of superoxide in blood vessels and impairs vasomotor function. Angiotensin II (AII) plays an important role in generation of superoxide in several disease states, including hypertension and heart failure. The goal of this study was to determine whether enalapril, an angiotensin‐converting enzyme inhibitor (ACEI), or losartan, an angiotensin receptor blocker (ARB), attenuates increases of superoxide and vasomotor dysfunction in mice treated with LPS. C57/BL/6 mice were treated with either enalapril (20mg/kg/day) or losartan (5 mg/kg/day) for 4 days. After the third day, LPS (E. coli stereotype 0128:B12, 10–20 mg kg) was injected i.p. One day later, vasomotor function of aorta was examined. After precontraction with PGF‐2∞, maximal responses to sodium nitroprusside (10−9 to 10−5) were similar in aorta from normal and LPS‐treated mice. In contrast, relaxation to acetylcholine (10−5) was impaired after LPS (54±5%, mean±SE) compared to normal vessels (88±1%; p<0.05). Enalapril improved (p<0.05) relaxation in response to acetylcholine to 81±6%. Losartan also improved relaxation in response to acetylcholine after LPS to 77±4%. Superoxide (measured with lucigenin and hydroethidine) was increased in aorta after LPS, and levels were reduced following enalapril and losartan. Thus, enalapril or losartan reduce superoxide levels and improve relaxation to acetylcholine in the aorta after LPS. The findings suggest that activation of the renin/angiotensin system contributes importantly to oxidative stress and endothelial dysfunction after endotoxin.
Details
- Title: Subtitle
- Role of angiotensin in endothelial dysfunction after lipopolysaccharide in mice
- Creators
- Donald D. Lund - University of IowaRobert M. Brooks - University of IowaFrank M. Faraci - University of IowaDonald D. Heistad - University of Iowa
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.20(5), pp.A1164-A1164
- Publisher
- Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.20.5.A1164-c
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Language
- English
- Date published
- 03/2006
- Academic Unit
- Neuroscience and Pharmacology; Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984304749002771
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