Shifts in surface composition of NCCL-affected dentin and enamel

A L Kraus; E Teixeira; H Sun

doi:10.1016/j.dental.2025.01.115

Purpose / Aim: The prevalence of noncarious cervical lesions (NCCLs) has increased in the adult population. This study investigated changes in the NCCL-affected enamel and dentin's composition as compared to sound tissue. Materials & Methods: Briefly, sound and NCCL teeth were cut on the long axis and exposed surface was chemically characterized with Raman spectroscopy. The spectra were recorded using a Raman microspectrometer (Renishaw Raman Microscope), with a 50x microscope objective and a 785 nm laser at 50% power. Raman signal was collected in the spectral interval 100 - 4200 cm-1, with 10 s scan time and 5 accumulations. Spectra was baseline corrected and smoothened with Savitzky-Golay filter. The relative peak intensities and peak area (integrated peak intensity) were used to calculate the following ratios: enamel mineral phase ratio for carbonate and phosphate (respectively, 1070 cm-1/958 cm-1); dentin’s mineral to matrix (958 cm-1/ 1451 cm-1), collagen posttranslational modifications (Amide I 1656 cm-1 / CH2 scissoring 1451 cm-1) and presence of proteoglycans (PGs at 1370 cm-1 / CH2 scissoring 1451 cm-1). Data was statistically analyzed by One-way Anova and Tukey’s (α = 0.05). Results: The mineral ratio was not different between sound and NCCL surrounding enamel (p = 0.745). There was an increased collagen posttranslational modification ratio (p = 0.01), which is representative of increased number of pyridinoline-rich cross-links, suggesting aging of type I collagen. Although numerically there is a trend of reduced PGs presence in the NCCL-affected dentin, it was not statistically significant (p = 0.098). There was no statistically significant difference in the mineral to matrix ratio between sound and NCCL-affected dentin (p = 0.223). Conclusions: Important changes within the extracellular matrix of the NCCL-affected dentin may likely contribute to lesion progression, decreased remineralization potential of the affected dentin and impair adhesion.

Shifts in surface composition of NCCL-affected dentin and enamel

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